LOCUS       Exported                3395 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  CinI expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    C332
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Alnahhas RN, Sadeghpour M, Chen Y, Frey AA, Ott W, Josic K, Bennett 
            MR
  TITLE     Majority sensing in synthetic microbial consortia.
  JOURNAL   Nat Commun. 2020 Jul 21;11(1):3659. doi: 10.1038/s41467-020-17475-z.
  PUBMED    32694598
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             65..94
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    229..245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    260..276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      1209..1236
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1339..2130)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     complement(2310..2327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2444..2989)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2524..2543)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3065..3084
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     protein_bind    3242..3258
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3247..3269
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 aaaggccccc aaggtagtta tccttaaaaa agccacagca tacatcctgt ccgtccaagc
       61 agaggagcaa aagctcattt ctgaagagga cttgttgcgg aaacgacgag aacagttgaa
      121 acacaaactt gaacagctac ggaactcttg tgcgtaagga aaagtaagga aaacgattcc
      181 ttctaacaga aatgtcctga gcaatcacct atgaactgtc gattgacatt gtgagcggat
      241 aacaagatac tgagcacaat tgtgagcgga taacaattgc atgccactta aaaaggagat
      301 caacaatgaa agcaattttc gtactgaaac atcttaatca tgcgggggag tgttactagt
      361 atgttcgtta tcattcaggc acatgagtat cagaaatacg ctgccgtact cgaccagatg
      421 tttcgtctgc gcaagaaggt cttcgccgat acgctctgct gggacgttcc tgtcatcggc
      481 ccttacgaac gtgacagcta cgattcgctt gctcccgcct atctcgtctg gtgcaacgac
      541 agccgcaccc gtctttatgg cggcatgcgc ctgatgccga cgaccggccc gacccttctc
      601 tacgacgtct tccgcgagac gttccctgat gccgccgatc ttatcgcccc cggcatctgg
      661 gaaggcacgc gcatgtgcat cgacgaggag gcgatcgcca aggatttccc cgagatcgac
      721 gccggccgcg ccttctccat gatgctgctc gcgctttgcg aatgcgcgct cgatcacggc
      781 atccacacga tgatctccaa ctacgagccc tacctcaagc gcgtctacaa gcgcgccggc
      841 gccgaggtgg aagaactcgg ccgcgcagac ggctacggca aatatcccgt ctgctgcggc
      901 gccttcgaag tctcggaccg cgtgctgcgc aagatgcgcg ccgccctcgg cctcacccta
      961 cccctttatg tcaggcacgt gccggcccgc tcggtcgtga cccaattcct ggagatggca
     1021 gcagcagcga acgacgaaaa ttacgccctt gcagcgtaat aaaagcttcc cctacgtgct
     1081 gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata ctatgactga
     1141 gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc gcaccgctgt
     1201 ctcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag agagaatata
     1261 aaaagccaga ttattaatcc ggctttttta ttatttccta gctagcgata tccgtcggct
     1321 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
     1381 gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
     1441 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
     1501 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
     1561 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
     1621 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
     1681 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
     1741 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
     1801 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
     1861 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
     1921 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
     1981 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
     2041 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
     2101 cgatacttcg gcgatcaccg cttccctcat gatgtttaac tttgttttag ggcgactgcc
     2161 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
     2221 ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagaagc
     2281 catgaaaacc gccggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt
     2341 cgactgcggc gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg
     2401 aagatactta acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc
     2461 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     2521 tataaagata ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct
     2581 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca
     2641 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     2701 tccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca
     2761 aaagcaccac tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg
     2821 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt
     2881 acctcggttc aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt
     2941 tttttcgttt tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct
     3001 tattaatcag ataaaatatt tctaggctca tgagcccgaa gtggcgagcc cgatcttccc
     3061 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     3121 ccacgatgcg tccggcgtag aggatctgct catgtttgac agcttatcga gctcagtgcg
     3181 ctcgagcttc ccaaccttac cagagggcgc cccagctggc aattccgacg tctgtgtgga
     3241 attgtgagcg gataacaatt tcacacaggg ccctcggaca ccgaggagaa tgtcaagagg
     3301 cgaacacaca acgtcttgga gcgccagagg aggaacgagc taaaacggag cttttttgcc
     3361 ctgcgtgacc agatcccgga gttggaaaac aatga
//
