LOCUS Exported 7175 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS ADAMTS5_pCSdest SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7175) AUTHORS Edie S, Zaghloul NA, Leitch CC, Klinedinst DK, Lebron J, Thole JF, McCallion AS, Katsanis N, Reeves RH TITLE Survey of Human Chromosome 21 Gene Expression Effects on Early Development in Danio rerio. JOURNAL G3 (Bethesda). 2018 Jul 2;8(7):2215-2223. doi: 10.1534/g3.118.200144. PUBMED 29760202 REFERENCE 2 (bases 1 to 7175) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7175 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..978 /label=CMV IE94 promoter /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" promoter 1013..1031 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1013..1030 /label=SP6 /note="SP6 promoter, forward primer" protein_bind 1085..1109 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(4137..4161) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" promoter complement(4183..4200) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 4205..4339 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4229..4248) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 4283..4302 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(4452..4472) /label=T3 /note="T3 promoter, forward primer" promoter complement(4452..4470) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(4491..4507) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4491..4507) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4504..4526) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4515..4531 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4539..4569) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4584..4605 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4722..4739) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4893..5481) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4973..4992) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5652..6512) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6275..6294 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6513..6617) /gene="bla" /label=AmpR promoter rep_origin complement(6643..7098) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6780..6801) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6992..7011 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 gccaattcaa tatggcgtat atggactcat gccaattcaa tatggtggat ctggacctgt 61 gccaattcaa tatggcgtat atggactcgt gccaattcaa tatggtggat ctggacccca 121 gccaattcaa tatggcggac ttggcaccat gccaattcaa tatggcggac ctggcactgt 181 gccaactggg gaggggtcta cttggcacgg tgccaagttt gaggaggggt cttggccctg 241 tgccaagtcc gccatattga attggcatgg tgccaataat ggcggccata ttggctatat 301 gccaggatca atatataggc aatatccaat atggccctat gccaatatgg ctattggcca 361 ggttcaatac tatgtattgg ccctatgcca tatagtattc catatatggg ttttcctatt 421 gacgtagata gcccctccca atgggcggtc ccatatacca tatatggggc ttcctaatac 481 cgcccatagc cactccccca ttgacgtcaa tggtctctat atatggtctt tcctattgac 541 gtcatatggg cggtcctatt gacgtatatg gcgcctcccc cattgacgtc aattacggta 601 aatggcccgc ctggctcaat gcccattgac gtcaatagga ccacccacca ttgacgtcaa 661 tgggatggct cattgcccat tcatatccgt tctcacgccc cctattgacg tcaatgacgg 721 taaatggccc acttggcagt acatcaatat ctattaatag taacttggca agtacattac 781 tattggaagt acgccagggt acattggcag tactcccatt gacgtcaatg gcggtaaatg 841 gcccgcgatg gctgccaagt acatccccat tgacgtcaat ggggaggggc aatgacgcaa 901 atgggcgttc cattgacgta aatgggcggt aggcgtgcct aatgggaggt ctatataagc 961 aatgctcgtt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt 1021 gacactatag aatacaagct acttgttctt tttgcaggat cccatcgatt cgaattcaag 1081 gatcacaagt ttgtacaaaa aagcaggctt taaaggaacc aattcagtcg actggatccg 1141 gtaccgaatt cgcggccgcg aattcgccct tatcccccgc cccgttaact tcatagcaaa 1201 taacaaatac ccataaagtc ccagtcgcgc agcccctccc cgcgggcagc gcactatgct 1261 gctcgggtgg gcgtccctgc tgctgtgcgc gttccgcctg cccctggccg cggtcggccc 1321 cgccgcgaca cctgcccagg ataaagccgg gcagcctccg actgctgcag cagccgccca 1381 gccccgccgg cggcaggggg aggaggtgca ggagcgagcc gagcctcccg gccacccgca 1441 ccccctggcg cagcggcgca ggagcaaggg gctggtgcag aacatcgacc aactctactc 1501 cggcggcggc aaggtgggct acctcgtcta cgcgggcggc cggaggttcc tcttggacct 1561 ggagcgagat ggttcggtgg gcattgctgg cttcgtgccc gcaggaggcg ggacgagtgc 1621 gccctggcgc caccggagcc actgcttcta tcggggcaca gtggacgcta gtccccgctc 1681 tctggctgtc tttgacctct gtgggggtct cgacggcttc ttcgcggtca agcacgcgcg 1741 ctacacccta aagccactgc tgcgcggacc ctgggcggag gaagaaaagg ggcgcgtgta 1801 cggggatggg tccgcacgga tcctgcacgt ctacacccgc gagggcttca gcttcgaggc 1861 cctgccgccg cgcgccagct gcgaaacccc cgcgtccaca ccggaggccc acgagcatgc 1921 tccggcgcac agcaacccga gcggacgcgc agcactggcc tcgcagctct tggaccagtc 1981 cgctctctcg cccgctgggg gctcaggacc gcagacgtgg tggcggcggc ggcgccgctc 2041 catctcccgg gcccgccagg tggagctgct tctggtggct gacgcgtcca tggcgcggtt 2101 gtatggccgg ggcctgcagc attacctgct gaccctggcc tccatcgcca ataggctgta 2161 cagccatgct agcatcgaga accacatccg cctggccgtg gtgaaggtgg tggtgctagg 2221 cgacaaggac aagagcctgg aagtgagcaa gaacgctgcc accacactca agaacttttg 2281 caagtggcag caccaacaca accagctggg agatgaccat gaggagcact acgatgcagc 2341 tatcctgttt actcgggagg atttatgtgg gcatcattca tgtgacaccc tgggaatggc 2401 agacgttggg accatatgtt ctccagagcg cagctgtgct gtgattgaag acgatggcct 2461 ccacgcagcc ttcactgtgg ctcacgaaat cggacattta cttggcctct cccatgacga 2521 ttccaaattc tgtgaagaga cctttggttc cacagaagat aagcgcttaa tgtcttccat 2581 ccttaccagc attgatgcat ctaagccctg gtccaaatgc acttcagcca ccatcacaga 2641 attcctggat gatggccatg gtaactgttt gctggaccta ccacgaaagc agatcctggg 2701 ccccgaagaa ctcccaggac agacctacga tgccacccag cagtgcaacc tgacattcgg 2761 gcctgagtac tccgtgtgtc ccggcatgga tgtctgtgct cgcctgtggt gtgctgtggt 2821 acgccagggc cagatggtct gtctgaccaa gaagctgcct gcggtggaag ggacgccttg 2881 tggaaagggg agaatctgcc tgcagggcaa atgtgtggac aaaaccaaga aaaaatatta 2941 ttcaacgtca agccatggca actggggatc ttggggatcc tggggccagt gttctcgctc 3001 atgtggagga ggagtgcagt ttgcctatcg tcactgtaat aaccctgctc ccagaaacaa 3061 cggacgctac tgcacaggga agagggccat ctaccgctcc tgcagtctca tgccctgccc 3121 acccaatggt aaatcatttc gtcatgaaca gtgtgaggcc aaaaatggct atcagtctga 3181 tgcaaaagga gtcaaaactt ttgtggaatg ggttcccaaa tatgcaggtg tcctgccagc 3241 ggatgtgtgc aagctgacct gcagagccaa gggcactggc tactatgtgg tattttctcc 3301 aaaggtgacc gatggcactg aatgtaggcc gtacagtaat tccgtctgcg tccgggggaa 3361 gtgtgtgaga actggctgtg acggcatcat tggctcaaag ctgcagtatg acaagtgcgg 3421 agtatgtgga ggagacaact ccagctgtac aaagattgtt ggaaccttta ataagaaaag 3481 taagggttac actgacgtgg tgaggattcc tgaaggggca acccacataa aagttcgaca 3541 gttcaaagcc aaagaccaga ctagattcac tgcctattta gccctgaaaa agaaaaacgg 3601 tgagtacctt atcaatggaa agtacatgat ctccacttca gagactatca ttgacatcaa 3661 tggaacagtc atgaactata gcggttggag ccacagggat gacttcctgc atggcatggg 3721 ctactctgcc acgaaggaaa ttctaatagt gcagattctt gcaacagacc ccactaaacc 3781 attagatgtc cgttatagct tttttgttcc caagaagtcc actccaaaag taaactctgt 3841 cactagtcat ggcagcaata aagtgggatc acacacttcg cagccgcagt gggtcacggg 3901 cccatggctc gcctgctcta ggacctgtga cacaggttgg cacaccagaa cggtgcagtg 3961 ccaggatgga aaccggaagt tagcaaaagg atgtcctctc tcccaaaggc cttctgcgtt 4021 taagcaatgc ttgttgaaga aatgttagcc tgtggttatg atcttatgca caaagataac 4081 tggaggattc agcaccgatg cagtcgtgta tataaagggc gaattcgttt atctagaccc 4141 agctttcttg tacaaagtgg tgatcctctc gagcctctag aactatagtg agtcgtatta 4201 cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 4261 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 4321 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 4381 ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtaccc 4441 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg 4501 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cgggagcata 4561 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 4621 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 4681 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 4741 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 4801 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 4861 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 4921 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 4981 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 5041 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 5101 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 5161 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 5221 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 5281 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 5341 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 5401 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 5461 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 5521 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 5581 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 5641 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 5701 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 5761 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 5821 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 5881 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 5941 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 6001 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 6061 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 6121 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 6181 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 6241 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 6301 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 6361 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 6421 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 6481 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 6541 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 6601 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata 6661 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 6721 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 6781 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 6841 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 6901 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 6961 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 7021 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 7081 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat 7141 cggtgcgggc ctcttcgcta ttacgccagt cgacc //