LOCUS       Exported                 648 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223-PRKD2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Nov 24. ():.
  PUBMED    21107320
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
       61 ttttttataa tgccaacttt gtacaaaaaa gttggcacca tggccaccgc cccctcttat
      121 cccgccgggc tccctggctc tcccgggccg gggtctcctc cgccccccgg cggcctagag
      181 ctgcagtcgc cgccaccgct actgccccag atcccggccc cgggttccgg ggtctccttt
      241 cacatccaga tcgggctgac ccgcgagttc gtgctgttgc ccgccgcctc cgagctggct
      301 catgtgaagc agctggcctg ttccatcgtg gaccagaagt tccctgagtg tggcttctac
      361 ggcctttacg acaagatcct gcttttcaaa catgacccca cgtcggccaa cctcctgcag
      421 ctggtgcgct cgtccggaga catccaggag ggcgacctgg tggaggtggt gctgtcggcc
      481 tcggccacct tcgaggactt ccagatccgc ccgcacgccc tcacggtgca ctcctatcgg
      541 gcgcctgcct tctgtgatca ctgcggggag atgctcttcg gcctagtgcg ccagggcctc
      601 aagtgcgatg gctgcgggct gaactaccac aagcgctgtg ccttcagc
//
