LOCUS       Exported                 665 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223-PFKFB3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Nov 24. ():.
  PUBMED    21107320
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    73..97
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc
       61 ttttttataa tgccaacttt gtacaaaaaa gttggcatgc cgttggaact gacgcagagc
      121 cgagtgcaga agatctgggt gcccgtggac cacaggccct cgttgcccag atcctgtggg
      181 ccaaagctga ccaactcccc caccgtcatc gtcatggtgg gcctccccgc ccggggcaag
      241 acctacatct ccaagaagct gactcgctac ctcaactgga ttggcgtccc cacaaaagtg
      301 ttcaacgtcg gggagtatcg ccgggaggct gtgaagcagt acagctccta caacttcttc
      361 cgccccgaca atgaggaagc catgaaagtc cggaagcaat gtgccttagc tgccttgaga
      421 gatgtcaaaa gctacctggc gaaagaaggg ggacaaattg cggttttcga tgccaccaat
      481 actactagag agaggagaca catgatcctt cattttgcca aagaaaatga ctttaaggcg
      541 tttttcatcg agtcggtgtg cgacgaccct acagttgtgg cctccaatat catggaagtt
      601 aaaatctcca gcccggatta caaagactgc aactcggcag aagccatgga cgacttcatg
      661 aagag
//
