LOCUS Exported 888 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression construct for producing Avi tagged Leucyl-tRNA synthetase. ACCESSION . VERSION . KEYWORDS LARS_pNIC-Bio3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 888) TITLE Graslund lab plasmids REFERENCE 2 (bases 1 to 888) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..888 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 4..21 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 46..66 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQS" CDS 844..888 /codon_start=1 /product="peptide tag that allows for enzymatic biotinylation" /label=AviTag(TM) /translation="GLNDIFEAQKIEWHE" ORIGIN 1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc 61 caatccatgg gacctcagga atatacttta ctcaaattga aggtgcttga gccataccca 121 tctaaattaa gtggcctgaa aggtaaaaat attttcttgg tggctgctac tctcagacct 181 gagaccatgt ttgggcagac aaattgttgg gttcgtcctg atatgaagta cattggattt 241 gagacggtga atggtgatat attcatctgt acccaaaaag cagccaggaa tatgtcatac 301 cagggcttta ccaaagacaa tggcgtggtg cctgttgtta aggaattaat gggggaggaa 361 attcttggtg catcactttc tgcaccttta acatcataca aggtgatcta tgttctccca 421 atgctaacta ttaaggagga taaaggcact ggtgtggtta caagtgttcc ttccgactcc 481 cctgatgata ttgctgccct cagagacttg aagaaaaagc aagccttacg agcaaaatat 541 ggaattagag atgacatggt cttgccattt gagccggtgc cagtcattga aatcccaggt 601 tttggaaatc tttctgctgt aaccatttgt gatgagttga aaattcagag ccagaatgac 661 cgggaaaaac ttgcagaagc aaaggagaag atatatctaa aaggatttta tgagggtatc 721 atgttggtgg atggatttaa aggacagaag gttcaagatg taaagaagac tattcagaaa 781 aagatgattg acgctggaga tgcacttatt tacatggaac cagagagcag taaaggtgga 841 tacggcctga atgatatctt tgaagcgcag aagattgaat ggcatgaa //