LOCUS Exported 10024 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2. ACCESSION . VERSION . KEYWORDS pAY19 (OsTIR1(F74A) mAID-EGFP-NLS) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10024) AUTHORS Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT. TITLE The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. JOURNAL Nat Commun (2020) 11(1):5701 REFERENCE 2 (bases 1 to 10024) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10024 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(144..165) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 356..375 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 588..610 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 602..619 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 603..619 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 629..648 /label=T7 /note="T7 promoter, forward primer" promoter 629..647 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 883..916 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(1473..2072) /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 2053..2072 /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" promoter complement(2112..2611) /label=PGK promoter /note="mouse phosphoglycerate kinase 1 promoter" primer_bind complement(2160..2179) /label=mPGK-F /note="Mouse PGK promoter, forward primer" primer_bind 2553..2576 /label=MSCV-rev /note="Murine stem cell virus, reverse primer" protein_bind 2672..2705 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 2732..2853 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2743..2762) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 2797..2816 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(3004..3024) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(3061..3777) /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS complement(3061..3777) /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3103..3124) /label=EGFP-C /note="EGFP, forward primer" primer_bind 3452..3471 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3711..3732 /label=EGFP-N /note="EGFP, reverse primer" CDS complement(3784..3987) /codon_start=1 /product="minimal degron from the auxin-responsive IAA17 protein of Arabidopsis thaliana (Kubota et al., 2013)" /label=mini-AID /note="acts as an auxin-inducible degron (AID) in the presence of TIR1" /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA AAFVKVSMDGAPYLRKIDLRMYK" CDS complement(3994..4050) /codon_start=1 /product="2A peptide from porcine teschovirus-1 polyprotein" /label=P2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="ATNFSLLKQAGDVEENPGP" primer_bind complement(5860..5879) /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" intron 5887..6903 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind complement(5941..5963) /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" promoter complement(6904..7181) /label=chicken beta-actin promoter enhancer 7183..7562 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind complement(7840..7860) /label=T3 /note="T3 promoter, forward primer" promoter complement(7840..7858) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7879..7895) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7879..7895) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7892..7914) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7903..7919 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7927..7957) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7972..7993 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8110..8127) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8281..8869) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8361..8380) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9040..9900) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9663..9682 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9901..10005) /gene="bla" /label=AmpR promoter ORIGIN 1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 361 agaaagcgaa aggagcgggc gctagggcgc ggcgggtgtg gtggtcacgc tgcgcgtaac 421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct 481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 601 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac 661 cggcatatgg ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt 721 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac 781 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag 841 taaaagcaag aaagatctta gtgaacctct tcgagggacc taataacttc gtatagcata 901 cattatacga agttatatta agggttccgg atctcgacca gcttctgatg gaattagaac 961 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctt 1021 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca 1081 agagaaaaac aatgatccca cgagagatct atagatctat agatcatgag tgggaggaat 1141 gagctggccc ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta 1201 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca 1261 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt 1321 cttaacaaag tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga 1381 tcatcaattt ctgcatcccg gggatctgat atcatcgatg catggggtcg tgcgctcctt 1441 tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac 1501 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg 1561 tagaagggga ggttgtgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg 1621 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc 1681 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg 1741 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg 1801 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg 1861 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt 1921 tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg 1981 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg 2041 gcgaggcgca ccgtgggctt gtactcggtc atggtaagct tcagctgctc gagatctaga 2101 tggatgcagg tcgaaaggcc cggagatgag gaagaggaga acagcgcggc agacgtgcgc 2161 ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc gggcgcccgc cccgcccctg 2221 agcccgcccc tgagcccgcc cccggaccca ccccttccca gcctctgagc ccagaaagcg 2281 aaggagcaaa gctgctattg gccgctgccc caaaggccta cccgcttcca ttgctcagcg 2341 gtgctgtcca tctgcacgag actagtgaga cgtgctactt ccatttgtca cgtcctgcac 2401 gacgcgagct gcggggcggg ggggaacttc ctgactaggg gaggagtaga aggtggcgcg 2461 aaggggccac caaagaacgg agtcggttgg cgcctaccgg tggatgtgga atgtgtgcga 2521 ggccagaggc cacttgtgta gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca 2581 gactgccttg ggaaaagcgc ctcccctacc cggtagaatt tcgaggtcga gatcctaagc 2641 ttggctggac gtaaactcct cttcagacct aataacttcg tatagcatac attatacgaa 2701 gttatattaa gggttattga atatgatcgc ttaagataca ttgatgagtt tggacaaacc 2761 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta 2821 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg 2881 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt 2941 ggtatggctg attatgatca gttatctaga tccggtggat cccgggcccg cggtaccgtc 3001 gatgaccttt cttttcttct tggggtcgac tgcagaattc gaagcttggg cgcctgcacc 3061 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac 3121 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg 3181 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc 3241 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt 3301 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg 3361 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag 3421 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa 3481 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt 3541 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt 3601 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta 3661 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc 3721 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatgct 3781 agctttatac atcctcaaat cgattttcct caagtacggt gctccgtcca ttgatacctt 3841 cacgaacgcc gccgcctccg ggccaccgct tgatttttgg caggaaacca tcacgttctt 3901 ccggtatgat ctcaccggtg gccatcccac aacttgtgcc ttggccggag gtttggctgg 3961 atctttagga caagcactct tctccttaga tctaggtcca gggttctcct ccacgtctcc 4021 agcctgcttc agcaggctga agttagtagc tccgcttcct gatctcagaa tcttcacaaa 4081 gttgggagca tcatctcttg cccctgcggt ggtccgatac acatacagtt tctccacctt 4141 tggcaggtcg ccatgatttt cctccatttc gttagacccg tcccgctcat taatgacttc 4201 cacgttcagc ataggcattt tactagccag gacctgacag cccttcagag tcacattgca 4261 actagacatc cacaggctcc tcattgtctc gtatctggca aagtttccca gcagagcggc 4321 atccccgaaa gggctgtccc ggatttccag ttttcgcaga ttcttacacc cgttcatcac 4381 gtgcatcatg cccttatcgg agtctccggc aaatgcaatg ctcagcatct ccagctgctt 4441 agcatacttg ccgatgtaca tgaacacttt gtctgtcagc aggccggaaa tgctcagcct 4501 ctggagtccc ttgcactctc tcacgatagc tccaaaccct tcatccagtg gctgggatgt 4561 gaccacgtcg ggtttgcctg gttccagaat acacagccga aatcgggtga aattagggca 4621 gttcttggcg acggtcacca gtgcagcatt agtcatctga tgacagaaat acagcaggct 4681 gttcagcttt gggcatccca gtgagactgc caccaggcct tcctcagtga cagcactata 4741 tccggccacg taaaaatcag atgggaacac tctcagctcc tggaggtcct tgcaggagct 4801 ggcgaccacc tggagcccct tatcggaaat acagtccagc acccacagtc gctggagttt 4861 cacgcagcgt gagatcatct ttgtcagatc actggcgtcc agggttggtg catagctcag 4921 gttcaggcct gtcagctgtg cacacagagg gtagataaag ctcaggcaca cgggtgaagc 4981 atcccagaat cctgacagac tcctcagcat tttacacttc tccagggcag aagtcagttt 5041 aaagtaggat tctgtctgga aatcgtctgt cagattgccg gttcccagat cctccaggtt 5101 aggggttctc agcagaatct tagccagagt gtccacgctc acgctccggt tcagccgcag 5161 agacctcagg ttggggcttc ttgagaccag ccgctccagg gacccagcat tcacttcgcc 5221 tttgatgcag gcaaagttca gggacaccag gctggtacat gagtctggga agcaagacag 5281 ccatctgggg cctctatcct ccacttcatt ctcctggagg tccagctccc gcagcagctt 5341 acagtgggag gccacggctg ccagtccatc ggtgctgaag ccttcgcaag aaatcagcac 5401 cagtgcgcga aacctaggga agctccgagc cagcagctcc agactttcgt cagagaccac 5461 cattcgcttc atgcgcagtt cctccagtcc atgacagccg cgagcggctg cctcgatcca 5521 agggccggcg tatcctcccc aatcaggggg caccaggtta aagtcggcgg cgtggggttt 5581 ccccttgacg gtcagtgcgc gcacatttgg aaacctagcg gcgaccctgc ctgctctcac 5641 agcatagcag ttgccgacga acactcctct ccggctcagg cgttcgatct cgtaccacac 5701 tttgcagacc aggctcactg tgtttctatc tctctgtgca ggcaggaagc taaaaatgtg 5761 ttcgacgacc tcttcaggaa agtatgtcat ggtggcagcg ctatctagat gcattcgcga 5821 ggtaccgagc tcgaattctt tgccaaaatg atgagacagc acaataacca gcacgttgcc 5881 caggagctgt aggaaaaaga agaaggcatg aacatggtta gcagaggctc tagagccgcc 5941 ggtcacacgc cagaagccga accccgccct gccccgtccc ccccgaaggc agccgtcccc 6001 ccgcggacag ccccgaggct ggagagggag aaggggacgg cggcgcggcg acgcacgaag 6061 gccctccccg cccatttcct tcctgccggc gccgcaccgc ttcgccccgc gcccgctaga 6121 gggggtgcgg cggcgcctcc cagatttcgg ctccgcacag atttgggaca aaggaagtcc 6181 ctgcgccctc tcgcacgatt accataaaag gcaatggctg cggctcgccg cgcctcgaca 6241 gccgccggcg ctccgggggc cgccgcgccc cycccccgag cccyccccgg cccgaggcgg 6301 ccccgccccg cccggcaccc ccacctgccg ccaccccccg cccggcacgg cgagccccgc 6361 gccacgcccc gtacggagcc ccgcacccga agccgggccg tgctcagcaa ctcggggagg 6421 ggggtgcagg gggggttgca gcccgaccga cgcgcccaca ccccctgctc acccccccac 6481 gcacacaccc cgcacgcagc ctttgttccc ctcgcagccc cccccgcacc gcggggcacc 6541 gcccccggcc gcgctcccct cgcgcacact gcggagcgca caaagccccg cgccgcgccc 6601 gcagcgctca cagccgccgg gcagcgcgga gccgcacgcg gcgctcccca cgcacacaca 6661 cacgcacgca ccccccgagc cgctcccccc gcacaaaggg ccctcccgga gcccctcaag 6721 gctttcacgc agccacagaa aagaaacaag ccgtcattaa accaagcgct aattacagcc 6781 cggaggagaa gggccgtccc gcccgctcac ctgtgggagt aacgcggtca gtcagagccg 6841 gggcgggcgg cgcgaggcgg cggcggagcg gggcacgggg cgaaggcagc gcgcagcgac 6901 tcccgcccgc cgcgcgcttc gctttttata gggccgccgc cgccgccgcc tcgccataaa 6961 aggaaacttt cggagcgcgc cgctctgatt ggctgccgcc gcacctctcc gcctcgcccc 7021 sccccscccc ycsccccscc ccsccccscc yggcgcgcgc cccccccccc cccccgcccc 7081 catcgctgca caaaataatt aaaaaataaa taaatacaaa attgggggtg gggagggggg 7141 ggagatgggg agagtgaagc agaacgtggg gctcacctcg accatggtaa tagcgatgac 7201 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg 7261 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac 7321 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa 7381 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc 7441 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata 7501 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg 7561 tcgagcatgc atctagaggg cccaagtgat ctccaaaaaa taagtacttt ttgactgtaa 7621 ataaaattgt aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt 7681 aaaagtattg atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa 7741 gtacagtaat caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc 7801 ttcagcaagc ccagcagatc cgagctccag cttttgttcc ctttagtgag ggttaattgc 7861 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7921 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7981 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 8041 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 8101 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 8161 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 8221 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 8281 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 8341 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 8401 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 8461 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 8521 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 8581 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 8641 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 8701 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 8761 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 8821 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 8881 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8941 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 9001 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 9061 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 9121 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 9181 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 9241 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 9301 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 9361 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 9421 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 9481 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 9541 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 9601 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 9661 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 9721 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 9781 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 9841 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9901 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9961 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 10021 agtg //