LOCUS       Exported                2919 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Gateway-compatible Entry vector, with insert of ORF7B gene's CDS 
            from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon. .
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223 SARS-CoV-2 ORF7B_nostop
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      37..64
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      156..242
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     291..313
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     305..322
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     306..322
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..437
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(567..666)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(683..702)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(684..702)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(707..723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(707..723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1154..1945
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2038..2626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2527..2546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2780..2797
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
       61 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
      121 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
      181 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
      241 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
      301 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact
      361 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
      421 gtacaaaaaa gttggcatga tcgagctgag cctgatcgac ttctacctgt gcttcctggc
      481 cttcctgctg ttcctggtgc tgatcatgct gatcatcttc tggttctccc tggagctgca
      541 ggaccacaac gaaacctgcc acgcttaccc aactttcttg tacaaagttg gcattataag
      601 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      661 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
      721 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
      781 atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa
      841 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
      901 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
      961 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     1021 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     1081 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     1141 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     1201 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     1261 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     1321 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     1381 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     1441 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     1501 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     1561 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     1621 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     1681 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1741 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1801 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1861 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     1921 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     1981 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2161 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2701 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2761 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2821 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2881 cgactggaaa gcgggcagtg agcgcaacgc aattaatac
//
