LOCUS       Exported                8725 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Mammalian Target-AIDmax expression vector (pSI-626).
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-Target-AIDmax
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8725)
  AUTHORS   Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto
            S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, 
            Nureki O, Tomita M, Aburatani H, Yachie N
  TITLE     Base editors for simultaneous introduction of C-to-T and A-to-G 
            mutations.
  JOURNAL   Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 
            10.1038/s41587-020-0509-0.
  PUBMED    32483365
REFERENCE   2  (bases 1 to 8725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8725
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        399..602
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     553..573
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     644..663
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        644..662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             712..732
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             733..4833
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5041..5106
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5740..5760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5767..6021
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     primer_bind     complement(6047..6064)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6053..6277
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6348..6364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6348..6364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6361..6383)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6372..6388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6396..6426)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6441..6462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6579..6596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6750..7338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6830..6849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7509..8369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8132..8151
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8370..8474)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8553..8572)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc catttacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      421 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      481 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      541 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      601 ctggtttagt gaaccgtcag atccgctaga gatccgcggc cgctaatacg actcactata
      661 gggagagccg ccaccatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag
      721 aagcggaaag tcgacaagaa gtacagcatc ggcctggcca tcggcaccaa ctctgtgggc
      781 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
      841 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
      901 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
      961 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     1021 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
     1081 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     1141 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
     1201 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
     1261 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     1321 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     1381 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     1441 ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     1501 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     1561 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     1621 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     1681 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
     1741 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     1801 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
     1861 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     1921 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
     1981 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     2041 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     2101 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     2161 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
     2221 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     2281 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     2341 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
     2401 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     2461 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     2521 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     2581 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
     2641 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
     2701 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
     2761 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
     2821 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
     2881 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
     2941 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
     3001 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
     3061 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
     3121 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
     3181 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
     3241 gtggaccata tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
     3301 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
     3361 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
     3421 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
     3481 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
     3541 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
     3601 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
     3661 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
     3721 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     3781 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
     3841 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
     3901 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
     3961 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
     4021 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
     4081 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
     4141 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
     4201 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
     4261 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
     4321 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
     4381 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
     4441 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
     4501 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
     4561 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
     4621 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
     4681 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
     4741 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
     4801 acacggatcg acctgtctca gctgggaggt gacggcggag gaggaactgg aggaggagga
     4861 tcagcagaat atgtgagagc actgtttgac tttaatggga atgacgaaga agacctgccc
     4921 ttcaagaagg gcgacatcct gcggatcaga gataagcctg aggagcagtg gtggaacgcc
     4981 gaggactctg agggcaagag aggcatgatc ctggtgccct acgtggagaa gtacagcggc
     5041 gattataagg accacgatgg cgactacaag gaccacgaca tcgactacaa ggatgacgat
     5101 gacaagtctg gcatgacaga tgccgagtat gtgagaatcc acgagaagct ggacatctac
     5161 accttcaaga agcagttttt caacaataag aagagcgtgt cccacagatg ctacgtgctg
     5221 tttgagctga agagaagggg cgagcggaga gcctgttttt ggggctacgc cgtgaacaag
     5281 ccccagtctg gcacagagcg gggcatccac gccgagatct tctccatccg gaaggtggag
     5341 gagtacctgc gggacaaccc aggccagttc accatcaact ggtacagctc ctggtcccca
     5401 tgtgccgact gtgccgagaa gatcctggag tggtacaacc aggagctgcg gggcaacggc
     5461 cacaccctga agatctgggc ctgcaagctg tactatgaga agaatgccag aaaccagatc
     5521 ggcctgtgga acctgagaga caatggcgtg ggcctgaacg tgatggtgtc tgagcactac
     5581 cagtgctgta ggaagatctt catccagtct tcccacaacc agctgaatga gaacagatgg
     5641 ctggagaaga cactgaagcg ggccgagaag tggagaagcg agctgtccat catgatccag
     5701 gtgaagatcc tgcacaccac caagtctcca gccgtgggcc caaagaagaa gcgcaaggtg
     5761 ggcaccatga ccaacctgtc tgacatcatc gagaaggaga caggcaagca gctggtaatc
     5821 caggagagca tcctgatgct gcctgaggag gtggaggagg taatcggcaa caagcctgag
     5881 agcgatatcc tggtgcacac cgcctatgat gagagcacag acgagaacgt gatgctgctg
     5941 accagcgacg cccctgagta caaaccttgg gcactggtca ttcaggatag taacggggag
     6001 aacaaaatca aaatgctgtg ataatgagtt taaacccgct gatcagcctc gactgtgcct
     6061 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6121 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6181 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6241 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6301 tggggctcga taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6361 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6421 tgtaaagcct agggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6481 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6541 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6601 tcggtcgttc tgctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6661 acagaatcat gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6721 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6781 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6841 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6901 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6961 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7021 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7081 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7141 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7201 ggtatctgcg ctctgctgaa gccagttacc ttctgaaaaa gagttggtag ctcttgatcc
     7261 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7321 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cactcagtgg
     7381 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7441 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7501 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7561 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7621 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7681 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7741 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7801 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7861 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7921 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7981 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8041 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8101 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8161 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8221 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8281 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8341 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8401 tatcagggtt attgtctcat gagcgtatac atatttgaat gtatttagaa aaataaacaa
     8461 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8521 cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc cgcatagtta
     8581 agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt
     8641 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
     8701 cgttttgcgc tgcttcgcga tgtac
//
