LOCUS       Exported                3423 bp ds-DNA     linear   SYN 09-JUL-2021
DEFINITION  Yeast Expression plasmid for SARS-CoV-2 leader protein.
ACCESSION   .
VERSION     .
KEYWORDS    pGBW-m4046362
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3423)
  TITLE     SARS-CoV-2 protein Yeast expression constructs, tagged, for protein 
            expression and/or purification
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        25..466
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    25..142
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     437..460
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1018..1035
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(2178..2196)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             2547..3350
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 gcggccgctt gctatattga agtacggatt agaagccgcc gagcgggcga cagccctccg
       61 acggaagact ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg
      121 tgcctcgcgc cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat
      181 gaagaggaaa aattggcagt aacctggccc cacaaacctt caaattaacg aatcaaatta
      241 acaaccatag gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag
      301 cgaagcgatg atttttgatc tattaacaga tatataaatg gaaaagctgc ataaccactt
      361 taactaatac tttcaacatt ttcagtttgt attacttctt attcaaatgt cataaaagta
      421 tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa aactataatg
      481 gaatctttgg tccctggatt taacgagaaa acccacgtac agctatccct tcccgtgttg
      541 caagttcgtg acgttctggt tcggggtttc ggtgattcag tcgaagaagt cttgagtgaa
      601 gccaggcaac atttaaagga tggtacttgt ggcttagttg aagtcgaaaa gggtgtgttg
      661 ccacaacttg agcaaccata cgttttcatt aagagatctg acgcaagaac agcgccacac
      721 ggtcatgtta tggttgaatt ggtcgctgaa ttggagggta tacaatatgg cagatcgggt
      781 gaaactttag gtgtattggt cccacacgta ggtgaaatcc ctgttgctta cagaaaagtt
      841 ttattaagaa agaatggtaa caagggtgct ggtggacact cctacggagc cgatctgaag
      901 tcttttgact tgggtgatga attgggtact gatccatatg aagacttcca agaaaattgg
      961 aacaccaaac atagctctgg tgttacgcgt gaattgatga gagaacttaa tggtggtctc
     1021 gtgccgagag gttctaaaat cgaggaaggt aagctagtca tttggataaa tggagataag
     1081 ggttacaacg gtttggcaga agtaggcaag aaatttgaaa aggacacagg tatcaaggtg
     1141 acggttgaac atccagacaa gttagaagaa aaattccctc aagttgctgc tactggtgat
     1201 ggtcccgaca ttattttctg ggcccacgat cgttttggtg gatatgctca gtcaggtttg
     1261 ctggctgaga tcaccccaga caaggccttc caagataaat tgtacccatt cacttgggat
     1321 gctgtcagat acaacggtaa gcttatagca tatccaattg ctgttgaagc cttatcgttg
     1381 atctacaata aggacttgtt acctaaccca ccaaagacat gggaagaaat tccagctttg
     1441 gataaagagc taaaggccaa gggaaaaagc gctttgatgt ttaacttaca agaaccttat
     1501 ttcacctggc cattaatcgc tgctgacggt ggttacgcat ttaaatacga aaacggtaag
     1561 tatgacatta aggatgtcgg cgttgataat gctggtgcaa aggctggttt gaccttcttg
     1621 gttgatttaa taaagaataa acacatgaac gctgacactg actactctat cgccgaagcc
     1681 gctttcaaca agggtgaaac tgctatgacc ataaatggtc catgggcctg gagtaacatt
     1741 gatacttcta aagtaaacta tggtgtcact gttttgccta cattcaaagg ccaaccatcc
     1801 aagccctttg tcggtgttct atccgctggc atcaatgctg cttctccaaa caaggagcta
     1861 gctaaagaat ttttggaaaa ttacttgtta actgacgaag gtttggaggc cgttaacaag
     1921 gacaagccat tgggtgctgt cgctttgaaa tcttacgaag aagaattggc taaggatccc
     1981 agaattgccg ctaccatgga gaacgcccaa aagggtgaaa ttatgccaaa catcccacaa
     2041 atgtcagcct tctggtacgc agttaggact gccgtgatta atgctgcttc cggtagacaa
     2101 accgtcgacg aagcattaaa agatgctcaa acgtaaacag gccccttttc ctttgtcgat
     2161 atcatgtaat tagttatgtc acgcttacat tcacgccctc ctcccacatc cgctctaacc
     2221 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt ttaatagtta
     2281 tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg tacaaagcgg
     2341 ccgccgaacc actagtgcac tgcagtacac ggtttccttg aaattttttt gattcggtaa
     2401 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca
     2461 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa
     2521 aaacgtgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta
     2581 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact
     2641 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag
     2701 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg
     2761 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaggaca
     2821 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa
     2881 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg
     2941 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag
     3001 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg
     3061 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag
     3121 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagatg
     3181 cattgggtca acagtataga accgtggatg atgtggtttc tacaggatct gacattatta
     3241 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca
     3301 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat
     3361 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
     3421 tat
//
