LOCUS Exported 3051 bp ds-DNA linear SYN 21-SEP-2021 DEFINITION Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase. ACCESSION . VERSION . KEYWORDS pGBW-m4046531 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3051) TITLE SARS-CoV-2 protein Yeast expression constructs, tagged, for protein expression and/or purification REFERENCE 2 (bases 1 to 3051) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3051 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 2866..2883 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" CDS 2884..2901 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(2946..2964) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" ORIGIN 1 tattacaact ttttttactt cttgctcatt agaaagaaag catagcaatc taatctaagt 61 tttaattaca aaatggctga tgcacaatcg tttttaaacc gggtttgcgg tgtaagtgca 121 gcccgtctta caccgtgcgg cacaggcact agtactgatg tcgtatacag ggcttttgac 181 atctacaatg ataaagtagc tggttttgct aaattcctaa aaactaattg ttgtcgcttc 241 caagaaaagg acgaagatga caatttaatt gattcttact ttgtagttaa gagacacact 301 ttctctaact accaacatga agaaacaatt tataatttac ttaaggattg tccagctgtt 361 gctaaacatg acttctttaa gtttagaata gacggtgaca tggtaccaca tatatcacgt 421 caacgtctta ctaaatacac aatggcagac ctcgtctatg ctttaaggca ttttgatgaa 481 ggtaattgtg acacattaaa agaaatactt gtcacataca attgttgtga tgatgattat 541 ttcaataaaa aggactggta tgattttgta gaaaacccag atatattacg cgtatacgcc 601 aacttaggtg aacgtgtacg ccaagctttg ttaaaaacag tacaattctg tgatgccatg 661 cgaaatgctg gtattgttgg tgtactgaca ttagataatc aagatctcaa tggtaactgg 721 tatgatttcg gtgatttcat acaaaccacg ccaggtagtg gagttcctgt tgtagattct 781 tattattcat tgttaatgcc tatattaacc ttgaccaggg ctttaactgc agagtcacat 841 gttgacactg acttaacaaa gccttacatt aagtgggatt tgttaaaata tgacttcacg 901 gaagagaggt taaaactctt tgaccgttat tttaaatatt gggatcagac ataccaccca 961 aattgtgtta actgtttgga tgacagatgc attctgcatt gtgcaaactt taatgtttta 1021 ttctctacag tgttcccacc tacaagtttt ggaccactag tgagaaaaat atttgttgat 1081 ggtgttccat ttgtagtttc aactggatac cacttcagag agctaggtgt tgtacataat 1141 caggatgtaa acttacatag ctctagactt agttttaagg aattacttgt gtatgctgct 1201 gaccctgcta tgcacgctgc ttctggtaat ctattactag ataaacgcac tacgtgcttt 1261 tcagtagctg cacttactaa caatgttgct tttcaaactg tcaaacccgg taattttaac 1321 aaagacttct atgactttgc tgtgtctaag ggtttcttta aggaaggaag ttctgttgaa 1381 ttaaaacact tcttctttgc tcaggatggt aatgctgcta tcagcgatta tgactactat 1441 cgttataatc taccaacaat gtgtgatatc agacaactac tatttgtagt tgaagttgtt 1501 gataagtact ttgattgtta cgatggtggc tgtattaatg ctaaccaagt catcgtcaac 1561 aacctagaca aatcagctgg ttttccattt aataaatggg gtaaggctag actttattat 1621 gattcaatga gttatgagga tcaagatgca cttttcgcat atacaaaacg taatgtcatc 1681 cctactataa ctcaaatgaa tcttaagtat gccattagtg caaagaatag agctcgcacc 1741 gtagctggtg tctctatctg tagtactatg accaatagac agtttcatca aaaattattg 1801 aaatcaatag ccgccactag aggagctact gtagtaattg gaacaagcaa attctatggt 1861 ggttggcaca acatgttaaa aactgtttat agtgatgtag aaaaccctca ccttatgggt 1921 tgggattatc ctaaatgtga tagagccatg cctaacatgc ttagaattat ggcctcactt 1981 gttcttgctc gcaaacatac aacgtgttgt agcttgtcac accgtttcta tagattagct 2041 aatgagtgtg ctcaagtatt gagtgaaatg gtcatgtgtg gcggttcact atatgttaaa 2101 ccaggtggaa cctcatcagg agatgccaca actgcttatg ctaatagtgt ttttaacatt 2161 tgtcaagctg tcacggccaa tgttaatgca cttttatcta ctgatggtaa caaaattgcc 2221 gataagtatg tccgcaattt acaacacaga ctttatgagt gtctctatag aaatagagat 2281 gttgacacag actttgtgaa tgagttttac gcatatttgc gtaaacattt ctcaatgatg 2341 atactctctg acgatgctgt tgtgtgtttc aatagcactt atgcatctca aggtctagtg 2401 gctagcataa agaactttaa gtcagttctt tattatcaaa acaatgtttt tatgtctgaa 2461 gcaaaatgtt ggactgagac tgaccttact aaaggacctc atgaattttg ctctcaacat 2521 acaatgctag ttaaacaggg tgatgattat gtgtaccttc cttacccaga tccatcaaga 2581 atcctagggg ccggctgttt tgtagatgat atcgtaaaaa cagatggtac acttatgatt 2641 gaacggttcg tgtctttagc tatagatgct tacccactta ctaaacatcc taatcaggag 2701 tatgctgatg tctttcattt gtacttacaa tacataagaa agctacatga tgagttaaca 2761 ggacacatgt tagacatgta ttctgttatg cttactaatg ataacacttc aaggtattgg 2821 gaacctgagt tttatgaggc tatgtacaca ccgcatacag tcttactcgt gccgagaggt 2881 tctcaccatc atcaccatca ttaaacaggc cccttttcct ttgtcgatat catgtaatta 2941 gttatgtcac gcttacattc acgccctcct cccacatccg ctctaaccga aaaggaagga 3001 gttagacaac ctgaagtcta ggtccctatt tatttttttt aatagttatg t //