LOCUS       Exported               14247 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for 
            creation of induced pluripotent stem cells.
ACCESSION   .
VERSION     .
KEYWORDS    PB-TET-MKOS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14247)
  AUTHORS   Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hamalainen
            R, Cowling R, Wang W, Liu P, Gertsenstein M, Kaji K, Sung HK, Nagy A
  TITLE     piggyBac transposition reprograms fibroblasts to induced pluripotent
            stem cells.
  JOURNAL   Nature. 2009 Mar 1. ():.
  PUBMED    19252478
REFERENCE   2  (bases 1 to 14247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14247
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    13..565
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(157..174)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     384..403
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             604..3645
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQ"
     CDS             3670..4473
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3733..3752)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4343..4362
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4505..4522)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4511..4735
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5174..5208
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(5357..5375)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5396..5412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5396..5412)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5409..5431)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5420..5436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5444..5474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5489..5510
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5627..5644)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5798..6386)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5878..5897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6557..7417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7180..7199
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7418..7522)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7548..8003)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7685..7706)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7897..7916
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8144..8161
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8145..8161
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8168..8187
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8168..8186
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   8253..8315
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    8687..8705
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8724..8742
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8761..8779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8798..8816
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8835..8853
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8872..8890
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8909..8927
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8946..8964
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     8986..9010
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    9090..9114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10487..10552
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             12011..12064
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             13136..13195
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     protein_bind    complement(14185..14209)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
       61 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
      121 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
      181 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
      241 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
      301 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
      361 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
      421 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
      481 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
      541 tcctttgaaa aacacgatga taatatggcc atgagcttga tgatctgtga catggcggat
      601 cccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      661 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      721 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
      781 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
      841 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
      901 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
      961 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
     1021 tcggcgtttc atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg
     1081 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
     1141 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc
     1201 attttccgtg acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt
     1261 gccactcgct ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc
     1321 ggcgagttgc gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc
     1381 gccagcggca ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat
     1441 cgcgtcacac tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat
     1501 ctctatcgtg cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc
     1561 tgcgatgtcg gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag
     1621 ccgttgctga ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg
     1681 gatgagcaga cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg
     1741 cgctgttcgc attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg
     1801 tatgtggtgg atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc
     1861 gatgatccgc gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat
     1921 cgtaatcacc cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat
     1981 cacgacgcgc tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa
     2041 ggcggcggag ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat
     2101 gaagaccagc ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct
     2161 ggagagacgc gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc
     2221 ggtttcgcta aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc
     2281 tgggactggg tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct
     2341 tacggcggtg attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc
     2401 tttgccgacc gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc
     2461 cagttccgtt tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc
     2521 gataacgagc tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa
     2581 gtgcctctgg atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag
     2641 ccggagagcg ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca
     2701 tggtcagaag ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt
     2761 gtgacgctcc ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt
     2821 tgcatcgagc tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag
     2881 atgtggattg gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca
     2941 ccgctggata acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc
     3001 gaacgctgga aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca
     3061 gatacacttg ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa
     3121 accttattta tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc
     3181 gttgatgttg aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag
     3241 ctggcgcagg tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc
     3301 gaccgcctta ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc
     3361 ccgtacgtct tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc
     3421 ccacaccagt ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg
     3481 atggaaacca gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac
     3541 ggtttccata tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc
     3601 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaggggat cccccgggct
     3661 gcagccaata tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct
     3721 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     3781 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     3841 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     3901 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     3961 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4021 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4081 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4141 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4201 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     4261 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     4321 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     4381 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     4441 gccttctatc gccttcttga cgagttcttc tgaggggatc aattctctag agctcgctga
     4501 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4561 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4621 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4681 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4741 gaggcggaaa gaaccagctg gggctcgatc ctctagagtc gagggctgca ggaattcgat
     4801 atcaagctct agccaacaag ctcgtcatcg ctttgcagaa gagcagagag gatatgctca
     4861 tcgtctaaag aactacccat tttattatat attagtcacg atatctataa caagaaaata
     4921 tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt atcgtatgag
     4981 ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac gcggtcgtta
     5041 tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga gctccaagcg
     5101 gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa gagagagcaa
     5161 tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt aaaaaagatt
     5221 tgcgctttac tcgacctaaa ctttaaacac gtcatagaat cttcgtttga caaaaaccac
     5281 attgtggcca agctgtgtga cgcgacgcgc gctaaagaat ggcaaaccaa gtcgcgcgag
     5341 gtaccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
     5401 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5461 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5521 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5581 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5641 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5701 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5761 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5821 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5881 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5941 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6001 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6061 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6121 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6181 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6241 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6301 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6361 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6421 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6481 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6541 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6601 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6661 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6721 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6781 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6841 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6901 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6961 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7021 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7081 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7141 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7201 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7261 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7321 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7381 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7441 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7501 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     7561 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     7621 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     7681 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     7741 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     7801 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     7861 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     7921 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7981 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     8041 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     8101 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     8161 tgaattgtaa tacgactcac tatagggcga attggagctc ggtattcacg acagcaggct
     8221 gaataataaa aaaattagaa actattattt aaccctagaa agataatcat attgtgacgt
     8281 acgttaaaga taatcatgcg taaaattgac gcatgtgttt tatcggtctg tatatcgagg
     8341 tttatttatt aatttgaata gatattaagt tttattatat ttacacttac atactaataa
     8401 taaattcaac aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa
     8461 aatttcttct ataaagtaac aaaactttta aacattctct cttttacaaa aataaactta
     8521 ttttgtactt taaaaacagt catgttgtat tataaaataa gtaattagct taacctatac
     8581 ataatagaaa caaattatac ttattagtca gtcagaaaca actttggcac atatcaatat
     8641 tatgctctcg ttaattaact cgagggatcc aagctggccg cacgtctccc tatcagtgat
     8701 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtacgt ctagaacgtc
     8761 tccctatcag tgatagagaa gtcgacacgt ctcgagctcc ctatcagtga tagagaaggt
     8821 acgtctagaa cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca
     8881 gtgatagaga aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct
     8941 cgagctccct atcagtgata gagaaggtac cccctatata agcagagctc gtttagtgaa
     9001 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
     9061 ccgatccagc ctccgcggtg gcggccatca caagtttgta caaaaaagca ggctggcgcc
     9121 ggaaccaatt cagtcgactg gatccggtac cgaattcacc atgcccctca acgtgaactt
     9181 caccaacagg aactatgacc tcgactacga ctccgtacag ccctatttca tctgcgacga
     9241 ggaagagaat ttctatcacc agcaacagca gagcgagctg cagccgcccg cgcccagtga
     9301 ggatatctgg aagaaattcg agctgcttcc caccccgccc ctgtccccga gccgccgctc
     9361 cgggctctgc tctccatcct atgttgcggt cgctacgtcc ttctccccaa gggaagacga
     9421 tgacggcggc ggtggcaact tctccaccgc cgatcagctg gagatgatga ccgagttact
     9481 tggaggagac atggtgaacc agagcttcat ctgcgatcct gacgacgaga ccttcatcaa
     9541 gaacatcatc atccaggact gtatgtggag cggtttctca gccgctgcca agctggtctc
     9601 ggagaagctg gcctcctacc aggctgcgcg caaagacagc accagcctga gccccgcccg
     9661 cgggcacagc gtctgctcca cctccagcct gtacctgcag gacctcaccg ccgccgcgtc
     9721 cgagtgcatt gacccctcag tggtctttcc ctacccgctc aacgacagca gctcgcccaa
     9781 atcctgtacc tcgtccgatt ccacggcctt ctctccttcc tcggactcgc tgctgtcctc
     9841 cgagtcctcc ccacgggcca gccctgagcc cctagtgctg catgaggaga caccgcccac
     9901 caccagcagc gactctgaag aagagcaaga agatgaggaa gaaattgatg tggtgtctgt
     9961 ggagaagagg caaacccctg ccaagaggtc ggagtcgggc tcatctccat cccgaggcca
    10021 cagcaaacct ccgcacagcc cactggtcct caagaggtgc cacgtctcca ctcaccagca
    10081 caactacgcc gcacccccct ccacaaggaa ggactatcca gctgccaaga gggccaagtt
    10141 ggacagtggc agggtcctga agcagatcag caacaaccgc aagtgctcca gccccaggtc
    10201 ctcagacacg gaggaaaacg acaagaggcg gacacacaac gtcttggaac gtcagaggag
    10261 gaacgagctg aagcgcagct tttttgccct gcgtgaccag atccctgaat tggaaaacaa
    10321 cgaaaaggcc cccaaggtag tgatcctcaa aaaagccacc gcctacatcc tgtccattca
    10381 agcagacgag cacaagctca cctctgaaaa ggacttattg aggaaacgac gagaacagtt
    10441 gaaacacaaa ctcgaacagc ttcgaaactc tggtgcaggt tctggcgtga aacagacttt
    10501 gaattttgac cttctcaagt tggcgggaga cgtggagtcc aacccagggc ccatgaggca
    10561 gccacctggc gagtctgaca tggctgtcag cgacgctctg ctcccgtcct tctccacgtt
    10621 cgcgtccggc ccggcgggaa gggagaagac actgcgtcca gcaggtgccc cgactaaccg
    10681 ttggcgtgag gaactctctc acatgaagcg acttccccca cttcccggcc gcccctacga
    10741 cctggcggcg acggtggcca cagacctgga gagtggcgga gctggtgcag cttgcagcag
    10801 taacaacccg gccctcctag cccggaggga gaccgaggag ttcaacgacc tcctggacct
    10861 agactttatc ctttccaact cgctaaccca ccaggaatcg gtggccgcca ccgtgaccac
    10921 ctcggcgtca gcttcatcct cgtcttcccc agcgagcagc ggccctgcca gcgcgccctc
    10981 cacctgcagc ttcagctatc cgatccgggc cgggggtgac ccgggcgtgg ctgccagcaa
    11041 cacaggtgga gggctcctct acagccgaga atctgcgcca cctcccacgg cccccttcaa
    11101 cctggcggac atcaatgacg tgagcccctc gggcggcttc gtggctgagc tcctgcggcc
    11161 ggagttggac ccagtataca ttccgccaca gcagcctcag ccgccaggtg gcgggctgat
    11221 gggcaagttt gtgctgaagg cgtctctgac cacccctggc agcgagtaca gcagcccttc
    11281 ggtcatcagt gttagcaaag gaagcccaga cggcagccac cccgtggtag tggcgcccta
    11341 cagcggtggc ccgccgcgca tgtgccccaa gattaagcaa gaggcggtcc cgtcctgcac
    11401 ggtcagccgg tccctagagg cccatttgag cgctggaccc cagctcagca acggccaccg
    11461 gcccaacaca cacgacttcc ccctggggcg gcagctcccc accaggacta cccctacact
    11521 gagtcccgag gaactgctga acagcaggga ctgtcaccct ggcctgcctc ttcccccagg
    11581 attccatccc catccggggc ccaactaccc tcctttcctg ccagaccaga tgcagtcaca
    11641 agtcccctct ctccattatc aagagctcat gccaccgggt tcctgcctgc cagaggagcc
    11701 caagccaaag aggggaagaa ggtcgtggcc ccggaaaaga acagccaccc acacttgtga
    11761 ctatgcaggc tgtggcaaaa cctataccaa gagttctcat ctcaaggcac acctgcgaac
    11821 tcacacaggc gagaaacctt accactgtga ctgggacggc tgtgggtgga aattcgcccg
    11881 ctccgatgaa ctgaccaggc actaccgcaa acacacaggg caccggccct ttcagtgcca
    11941 gaagtgcgac agggcctttt ccaggtcgga ccaccttgcc ttacacatga agaggcactt
    12001 tggctccgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg
    12061 cccactcgag atggctggac acctggcttc agacttcgcc ttctcacccc caccaggtgg
    12121 gggtgatggg tcagcagggc tggagccggg ctgggtggat tctcgaacct ggctaagctt
    12181 ccaagggcct ccaggtgggc ctggaatcgg accaggctca gaggtattgg ggatctcccc
    12241 atgtccgccc gcatacgagt tctgcggagg gatggcatac tgtggacctc aggttggact
    12301 gggcctagtc ccccaagttg gcgtggagac tttgcagcct gagggccagg caggagcacg
    12361 agtggaaagc aactcagagg gaacctcctc tgagccctgt gccgaccgcc ccaatgccgt
    12421 gaagttggag aaggtggaac caactcccga ggagtcccag gacatgaaag ccctgcagaa
    12481 ggagctagaa cagtttgcca agctgctgaa gcagaagagg atcaccttgg ggtacaccca
    12541 ggccgacgtg gggctcaccc tgggcgttct ctttggaaag gtgttcagcc agaccaccat
    12601 ctgtcgcttc gaggccttgc agctcagcct taagaacatg tgtaagctgc ggcccctgct
    12661 ggagaagtgg gtggaggaag ccgacaacaa tgagaacctt caggagatat gcaaatcgga
    12721 gaccctggtg caggcccgga agagaaagcg aactagcatt gagaaccgtg tgaggtggag
    12781 tctggagacc atgtttctga agtgcccgaa gccctcccta cagcagatca ctcacatcgc
    12841 caatcagctt gggctagaga aggatgtggt tcgagtatgg ttctgtaacc ggcgccagaa
    12901 gggcaaaaga tcaagtattg agtattccca acgagaagag tatgaggcta cagggacacc
    12961 tttcccaggg ggggctgtat cctttcctct gcccccaggt ccccactttg gcaccccagg
    13021 ctatggaagc ccccacttca ccacactcta ctcagtccct tttcctgagg gcgaggcctt
    13081 tccctctgtt cccgtcactg ctctgggctc tcccatgcat tcaaacgggt cgggtcaatg
    13141 tactaactac gctttgttga aactcgctgg cgatgttgaa agtaaccccg gtcctatgta
    13201 taacatgatg gagacggagc tgaagccgcc gggcccgcag caagcttcgg ggggcggcgg
    13261 cggaggaggc aacgccacgg cggcggcgac cggcggcaac cagaagaaca gcccggaccg
    13321 cgtcaagagg cccatgaacg ccttcatggt atggtcccgg gggcagcggc gtaagatggc
    13381 ccaggagaac cccaagatgc acaactcgga gatcagcaag cgcctgggcg cggagtggaa
    13441 acttttgtcc gagaccgaga agcggccgtt catcgacgag gccaagcggc tgcgcgctct
    13501 gcacatgaag gagcacccgg attataaata ccggccgcgg cggaaaacca agacgctcat
    13561 gaagaaggat aagtacacgc ttcccggagg cttgctggcc cccggcggga acagcatggc
    13621 gagcggggtt ggggtgggcg ccggcctggg tgcgggcgtg aaccagcgca tggacagcta
    13681 cgcgcacatg aacggctgga gcaacggcag ctacagcatg atgcaggagc agctgggcta
    13741 cccgcagcac ccgggcctca acgctcacgg cgcggcacag atgcaaccga tgcaccgcta
    13801 cgacgtcagc gccctgcagt acaactccat gaccagctcg cagacctaca tgaacggctc
    13861 gcccacctac agcatgtcct actcgcagca gggcaccccc ggtatggcgc tgggctccat
    13921 gggctctgtg gtcaagtccg aggccagctc cagccccccc gtggttacct cttcctccca
    13981 ctccagggcg ccctgccagg ccggggacct ccgggacatg atcagcatgt acctccccgg
    14041 cgccgaggtg ccggagcccg ctgcgcccag tagactgcac atggcccagc actaccagag
    14101 cggcccggtg cccggcacgg ccattaacgg cacactgccc ctgtcgcaca tgtgagaatt
    14161 cgcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtg atggccgctc
    14221 tagctagtcg ataagctaat tccgccc
//
