LOCUS Exported 5316 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Ras GTPase, Excitation: 487, Emission: 509. ACCESSION . VERSION . KEYWORDS mEmerald-Rab7a-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5316) TITLE Davidson mEmerald REFERENCE 2 (bases 1 to 5316) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5316 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 119..422 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 423..626 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 577..597 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 665..674 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 671..1387 /codon_start=1 /product="brightly fluorescent monomeric variant of GFP" /label=mEmerald /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(716..737) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(977..996) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1324..1345 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 2162..2283 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2199..2218) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2253..2272 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2290..2745) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2427..2448) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2639..2658 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2772..2876 /gene="bla" /label=AmpR promoter promoter 2878..3235 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2901..2921) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3086..3221 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3148..3167 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3270..4064 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3324..3343) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3934..3953 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4252..4271) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4296..4343 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4672..5260 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5161..5180 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta 61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa 301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca 361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca 421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat 481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg 541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac 601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc 661 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 721 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 781 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 841 ctggcccacc ctcgtgacca ccttgaccta cggcgtgcag tgcttcgccc gctaccccga 901 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 961 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 1021 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 1081 cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa 1141 gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt 1201 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 1261 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga 1321 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 1381 gtacaagtcc ggactcagat ctcgagccat gacctctagg aagaaagtgt tgctgaaggt 1441 tatcatcctg ggagattctg gagtcgggaa gacatcactc atgaaccagt atgtgaataa 1501 gaaattcagc aatcagtaca aagccacaat aggagctgac tttctgacca aggaggtgat 1561 ggtggatgac aggctagtca caatgcagat atgggacaca gcaggacagg aacggttcca 1621 gtctctcggt gtggccttct acagaggtgc agactgctgc gttctggtat ttgatgtgac 1681 tgcccccaac acattcaaaa ccctagatag ctggagagat gagtttctca tccaggccag 1741 tccccgagat cctgaaaact tcccatttgt tgtgttggga aacaagattg acctcgaaaa 1801 cagacaagtg gccacaaagc gggcacaggc ctggtgctac agcaaaaaca acattcccta 1861 ctttgagacc agtgccaagg aggccatcaa cgtggagcag gcgttccaga cgattgcacg 1921 gaatgcactt aagcaggaaa cggaggtgga gctgtacaac gaatttcctg aacctatcaa 1981 actggacaag aatgaccggg ccaaggcctc ggcagaaagc tgcagttgct gaggatccac 2041 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt 2101 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 2161 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 2221 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 2281 ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa 2341 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat 2401 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg 2461 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac 2521 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta 2581 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag 2641 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg 2701 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc 2761 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 2821 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg 2881 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 2941 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 3001 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 3061 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 3121 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 3181 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg 3241 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt 3301 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 3361 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 3421 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg 3481 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 3541 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 3601 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 3661 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 3721 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 3781 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 3841 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 3901 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 3961 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 4021 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt 4081 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc 4141 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca 4201 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg 4261 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac 4321 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt 4381 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc 4441 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc 4501 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt 4561 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga 4621 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 4681 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 4741 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 4801 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 4861 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 4921 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 4981 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 5041 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 5101 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 5161 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 5221 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 5281 tttacggttc ctggcctttt gctggccttt tgctca //