LOCUS       Exported                 720 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pET28 Set9 wt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Nishioka K, Chuikov S, Sarma K, Erdjument-Bromage H, Allis CD, 
            Tempst P, Reinberg D
  TITLE     Set9, a novel histone H3 methyltransferase that facilitates 
            transcription by precluding histone tail modifications required for 
            heterochromatin formation.
  JOURNAL   Genes Dev. 2002 Feb 15. 16(4):479-89.
  PUBMED    11850410
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             56..73
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             83..100
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attccctctg aatattttgt ttactttaag aaggagatat accatgggca gcagccatca
       61 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatggata gcgacgacga
      121 gatggtggag gaggcggtgg aagggcacct ggacgatgac ggattaccgc acgggttctg
      181 cacagtcacc tactcctcca cagacagatt tgaggggaac tttgttcacg gagaaaagaa
      241 cggacggggg aagttcttct tctttgatgg cagcaccctg gaggggtatt atgtggatga
      301 tgccttgcag ggccagggag tttacactta cgaagatggg ggagttctcc agggcacgta
      361 tgtagacgga gagctgaacg gtccagccca ggaatatgac acagatggga gactgatctt
      421 caaggggcag tataaagata acattcgtca tggagtgtgc tggatatatt acccagatgg
      481 aggaagcctt gtaggagaag taaatgaaga tggggagatg actggagaga agatagccta
      541 tgtgtaccct gatgagagga ccgcacttta tgggaaattt attgatggag agatgataga
      601 aggcaaactg gctaccctta tgtccactga agaagggagg cctcactttg aactgatgcc
      661 tggaaattca gtgtaccact ttgataagtc gacttcatct tgcatttcta ccaatgctct
//
