LOCUS Exported 5517 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of Flag tagged human FOXA2 in mammalian cells. ACCESSION . VERSION . KEYWORDS Flag-FOXA2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5517) AUTHORS Moparthi L, Koch S TITLE A uniform expression library for the exploration of FOX transcription factor biology JOURNAL Elsevier 2020 REFERENCE 2 (bases 1 to 5517) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5517 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 35..53 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 35..52 /label=SP6 /note="SP6 promoter, forward primer" regulatory 85..94 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 94..117 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" regulatory 298..307 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(1547..1564) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 1569..1703 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1593..1612) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1647..1666 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1816..1836) /label=T3 /note="T3 promoter, forward primer" promoter complement(1816..1834) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(1855..1871) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1855..1871) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1868..1890) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1879..1895 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1903..1933) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1948..1969 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(2086..2103) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2257..2845) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2337..2356) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3016..3876) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3639..3658 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3877..3981) /gene="bla" /label=AmpR promoter rep_origin complement(4007..4462) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4144..4165) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4356..4375 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4540..5517 /label=CMV IE94 promoter /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" ORIGIN 1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag 61 ctacttgttc tttttgcagg atcagccacc atggattaca aggacgacga cgataaggga 121 tcccatcgat tcgaattcca ctcggcttcc agtatgctgg gagcggtgaa gatggaaggg 181 cacgagccgt ccgactggag cagctactat gcagagcccg agggctactc ctccgtgagc 241 aacatgaacg ccggcctggg gatgaacggc atgaacacgt acatgagcat gtcggcggcc 301 gccatgggca gcggctcggg caacatgagc gcgggctcca tgaacatgtc gtcgtacgtg 361 ggcgctggca tgagcccgtc cctggcgggg atgtcccccg gcgcgggcgc catggcgggc 421 atgggcggct cggccggggc ggccggcgtg gcgggcatgg ggccgcactt gagtcccagc 481 ctgagcccgc tcggggggca ggcggccggg gccatgggcg gcctggcccc ctacgccaac 541 atgaactcca tgagccccat gtacgggcag gcgggcctga gccgcgcccg cgaccccaag 601 acctacaggc gcagctacac gcacgcaaag ccgccctact cgtacatctc gctcatcacc 661 atggccatcc agcagagccc caacaagatg ctgacgctga gcgagatcta ccagtggatc 721 atggacctct tccccttcta ccggcagaac cagcagcgct ggcagaactc catccgccac 781 tcgctctcct tcaacgactg tttcctgaag gtgccccgct cgcccgacaa gcccggcaag 841 ggctccttct ggaccctgca ccctgactcg ggcaacatgt tcgagaacgg ctgctacctg 901 cgccgccaga agcgcttcaa gtgcgagaag cagctggcgc tgaaggaggc cgcaggcgcc 961 gccggcagcg gcaagaaggc ggccgccgga gcccaggcct cacaggctca actcggggag 1021 gccgccgggc cggcctccga gactccggcg ggcaccgagt cgcctcactc gagcgcctcc 1081 ccgtgccagg agcacaagcg agggggcctg ggagagctga aggggacgcc ggctgcggcg 1141 ctgagccccc cagagccggc gccctctccc gggcagcagc agcaggccgc ggcccacctg 1201 ctgggcccgc cccaccaccc gggcctgccg cctgaggccc acctgaagcc ggaacaccac 1261 tacgccttca accacccgtt ctccatcaac aacctcatgt cctcggagca gcagcaccac 1321 cacagccacc accaccacca gccccacaaa atggacctca aggcctacga acaggtgatg 1381 cactaccccg gctacggttc ccccatgcct ggcagcttgg ccatgggccc ggtcacgaac 1441 aaaacgggcc tggacgcctc gcccctggcc gcagatacct cctactacca gggggtgtac 1501 tcccggccca ttatgaactc ctcttaagaa gacgacggct ctagaactat agtgagtcgt 1561 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa 1621 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca 1681 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc 1741 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt 1801 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata 1861 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccgggag 1921 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 1981 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 2041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 2101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 2161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 2221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 2281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 2341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 2401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 2461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 2521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 2581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 2641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 2701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 2761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 2821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 2881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 2941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 3001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 3061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 3121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 3181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 3241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 3301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 3361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 3421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 3481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 3541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 3601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 3661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 3721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 3781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 3841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 3901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 3961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt 4021 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 4081 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 4141 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 4201 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 4261 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 4321 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 4381 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 4441 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg 4501 cgatcggtgc gggcctcttc gctattacgc cagtcgaccg ccaattcaat atggcgtata 4561 tggactcatg ccaattcaat atggtggatc tggacctgtg ccaattcaat atggcgtata 4621 tggactcgtg ccaattcaat atggtggatc tggaccccag ccaattcaat atggcggact 4681 tggcaccatg ccaattcaat atggcggacc tggcactgtg ccaactgggg aggggtctac 4741 ttggcacggt gccaagtttg aggaggggtc ttggccctgt gccaagtccg ccatattgaa 4801 ttggcatggt gccaataatg gcggccatat tggctatatg ccaggatcaa tatataggca 4861 atatccaata tggccctatg ccaatatggc tattggccag gttcaatact atgtattggc 4921 cctatgccat atagtattcc atatatgggt tttcctattg acgtagatag cccctcccaa 4981 tgggcggtcc catataccat atatggggct tcctaatacc gcccatagcc actcccccat 5041 tgacgtcaat ggtctctata tatggtcttt cctattgacg tcatatgggc ggtcctattg 5101 acgtatatgg cgcctccccc attgacgtca attacggtaa atggcccgcc tggctcaatg 5161 cccattgacg tcaataggac cacccaccat tgacgtcaat gggatggctc attgcccatt 5221 catatccgtt ctcacgcccc ctattgacgt caatgacggt aaatggccca cttggcagta 5281 catcaatatc tattaatagt aacttggcaa gtacattact attggaagta cgccagggta 5341 cattggcagt actcccattg acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta 5401 catccccatt gacgtcaatg gggaggggca atgacgcaaa tgggcgttcc attgacgtaa 5461 atgggcggta ggcgtgccta atgggaggtc tatataagca atgctcgttt agggaac //