LOCUS Exported 2487 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0071 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2487) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2487) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2487 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 474..493 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 721..739 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 743..786 /label=bacterial terminator /note="putative bacterial transcription terminator" promoter 883..1228 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" primer_bind 1171..1191 /label=35S promoter /note="CaMV 35S promoter, forward primer" terminator 1249..1306 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1330..1347) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1501..2089) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1581..1600) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2272..2366 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2387..559) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga 61 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg 121 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt 181 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac 241 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac 301 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca 361 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg 421 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg 481 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc 541 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa 601 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga 661 tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 721 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat 781 gatttctgga attcggtctc aggaggtcaa catggtggag cacgacactc tggtctactc 841 caaaaatgtc aaagatacag tctcagaaga tcaaagggct attgagactt ttcaacaaag 901 gataatttcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag 961 gacagtagaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggctat 1021 cattcaagat ctctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat 1081 cgtggaaaaa gaagaggttc caaccacgtc tacaaagcaa gtggattgat gtgacatctc 1141 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata 1201 aggaagttca tttcatttgg agaggacacg ctactcgaga ccctgcagtc cggcaaaaaa 1261 gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat 1321 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 1381 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 1441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 1501 tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 1561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 1621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 1681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 1741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 1801 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 1861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 1921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 1981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 2041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 2101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 2161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 2221 atcaatctaa agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc 2281 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt 2341 cattactgga tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct 2401 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 2461 aaacggcatg atgaacctga atcgcca //