LOCUS Exported 2472 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0281 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2472) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2472) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2472 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 272..291 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 519..537 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 541..584 /label=bacterial terminator /note="putative bacterial transcription terminator" primer_bind 954..974 /label=35S promoter /note="CaMV 35S promoter, forward primer" promoter 963..1009 /label=minimal CaMV 35S promoter /note="minimal 35S promoter from cauliflower mosaic virus" terminator 1032..1089 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1113..1130) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1284..1872) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1364..1383) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2055..2149 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2170..357) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc 61 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc 121 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat 181 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc 241 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc 301 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt 361 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat 421 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg 481 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga 541 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcggtctcag 601 gaggtcaaca tggtggagca cgacactctg gtctactcca aaaatgtcaa agatacagtc 661 tcagaagatc aaagggctat tgagactttt caacaaagga taatttcggg aaacctcctc 721 ggattccatt gcccagctat ctgtcacttc atcgaaagga cagtagaaaa ggaaggtggc 781 tcctacaaat gccatcattg cgataaagga aaggctatca ttcaagatct ctctgccgac 841 agtggtccca aagatggacc cccacccacg aggagcatcg ttccaaccac gtctacaaag 901 caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc ccactatcct 961 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctactcg 1021 agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa 1081 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc 1141 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 1201 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 1261 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa 1321 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 1381 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 1441 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 1501 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 1561 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 1621 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 1681 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 1741 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 1801 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 1861 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 1921 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 1981 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 2041 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac 2101 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc 2161 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat 2221 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag 2281 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc 2341 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa 2401 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac 2461 atcttgcgaa ta //