LOCUS Exported 2491 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0290 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2491) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2491) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2491 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 299..318 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 546..564 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 568..611 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 1078..1135 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1159..1176) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1330..1918) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1410..1429) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2101..2195 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2216..384) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta 61 ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg 121 aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc 181 attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt 241 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc 301 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt 361 atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc 421 aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg 481 cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct 541 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta 601 aggatgattt ctggaattcg gtctcaggag tggaagattc agaaaatctc catcaacaaa 661 taatccaagt aaggattaat ggattgatca acatccttac cgctatgggt aagattgatg 721 aaaagtcaaa aacaaaaatc aattatattg tgggacacca atttcgtcca cagacatcaa 781 catcttatcg tcctttgaag ataagataat aatgttgaag ataagagtgg gagccaccac 841 taaaacattg ctttgtcaaa agctaaaaaa gatgatgccc gacagccact tgtgtgaagc 901 atgtgaagcc ggtccctcca ctaagaaaat tagtgaagca tcttccagtg gtccctccac 961 tcacagctca atcagtgagc aacaggacga aggaaatgac gtaagccatg acgtctaatc 1021 ccacaagaat ttccttatat aaggaacaca aatcagaagg tactcgagac cctgcagtcc 1081 ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga aaagattact 1141 tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 1201 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 1261 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 1321 gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 1381 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 1441 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 1501 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 1561 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 1621 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 1681 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 1741 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 1801 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 1861 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 1921 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 1981 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 2041 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct cgaggcttgg 2101 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag 2161 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg 2221 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga 2281 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt 2341 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt 2401 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa 2461 taaacccttt agggaaatag gccaggtttt c //