LOCUS Exported 2501 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0286 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2501) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2501) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2501 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 155..173 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 177..220 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 697..754 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(778..795) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(949..1537) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1029..1048) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1720..1814 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1835..2494) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2409..2428 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" ORIGIN 1 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca 61 ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta 121 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt 181 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgg tctcaggagt 241 ggaagattca gaaaatctcc atcaacaaat aatccaagta aggattaatg gattgatcaa 301 caagattgat gaaaagtcaa aaacaaaaat caattatgca caccagcatg tgttgatcac 361 cagctattgt gggacaccaa tttcgtccac agacatcaac atcttatcgt cctttgaaga 421 taagataata atgttgaaga taagagtggg agccaccact aaaacattgc tttgtcaaaa 481 gctaaaaaag atgatgcccg acagccactt gtgtgaagca tgtgaagccg gtccctccac 541 taagaaaatt agtgaagcat cttccagtgg tccctccact cacagctcaa tcagtgagca 601 acaggacgaa ggaaatgacg taagccatga cgtctaatcc cacaagaatt tccttatata 661 aggaacacaa atcagaaggt actcgagacc ctgcagtccg gcaaaaaagg gcaaggtgtc 721 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc 781 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 841 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 901 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct 961 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 1021 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 1081 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 1141 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 1201 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 1261 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 1321 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 1381 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 1441 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 1501 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 1561 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 1621 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 1681 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc 1741 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc 1801 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcatcgc 1861 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat 1921 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg 1981 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac 2041 tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg 2101 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat 2161 cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt 2221 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt 2281 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct 2341 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg 2401 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat 2461 caacggtggt atatccagtg atttttttct ccattttagc t //