LOCUS Exported 2483 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0291 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2483) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2483) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2483 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 310..329 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 557..575 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 579..622 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 1081..1138 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1162..1179) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1333..1921) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1413..1432) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2104..2198 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2219..395) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa 61 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg 121 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat 181 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc 241 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag 301 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata 361 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat 421 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa 481 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat 541 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt 601 agctttcgct aaggatgatt tctggaattc ggtctcagga gtggaagatt cagaaaatct 661 ccatcaacaa ataatccaag taaggattaa tggattgatc aacatcctta ccgctatggg 721 tgcacaccag catgtgttga tcaccagcta ttgtgggaca ccaatttcgt ccacagacat 781 caacatctta tcgtcctttg aagataagat aataatgttg aagataagag tgggagccac 841 cactaaaaca ttgctttgtc aaaagctaaa aaagatgatg cccgacagcc acttgtgtga 901 agcatgtgaa gccggtccct ccactaagaa aattagtgaa gcatcttcca gtggtccctc 961 cactcacagc tcaatcagtg agcaacagga cgaaggaaat gacgtaagcc atgacgtcta 1021 atcccacaag aatttcctta tataaggaac acaaatcaga aggtactcga gaccctgcag 1081 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt 1141 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 1201 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 1261 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 1321 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc 1381 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 1441 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 1501 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 1561 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 1621 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 1681 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 1741 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 1801 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 1861 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 1921 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 1981 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 2041 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct 2101 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg 2161 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt 2221 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat 2281 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc 2341 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca 2401 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct 2461 caataaaccc tttagggaaa tag //