LOCUS Exported 2311 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0294 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2311) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2311) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2311 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 178..196 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 200..243 /label=bacterial terminator /note="putative bacterial transcription terminator" promoter 313..492 /label=NOS promoter /note="nopaline synthase promoter" terminator 530..587 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(611..628) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(782..1370) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(862..881) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1553..1647 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1668..16) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2242..2261 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" ORIGIN 1 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata 61 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca 121 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 181 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat 241 ttctggaatt cggtctcagg agagcggaga attaagggag tcacgcaagc cgttttacgt 301 ttggaactga cagaaccgca acgttgaagg agccactcag ccgcgggttt ctggagttta 361 atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac 421 tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc 481 ggtatccaat tagagtctca tattcactct catactcgag accctgcagt ccggcaaaaa 541 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta 601 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 781 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 1021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 1081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 1141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 1201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 1261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 1321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 1381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 1441 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 1501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac 1561 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg 1621 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc 1681 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca 1741 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa 1801 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca 1861 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct 1921 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga 1981 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca 2041 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt 2101 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga 2161 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg 2221 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc 2281 cattgggata tatcaacggt ggtatatcca g //