LOCUS Exported 2323 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0293 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2323) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2323) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2323 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 200..219 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 447..465 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 469..512 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 811..868 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(892..909) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1063..1651) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1143..1162) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1834..1928 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1949..285) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc 61 catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata 121 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt 181 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca 241 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc 301 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg 361 aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca 421 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa 481 aaaaatcctt agctttcgct aaggatgatt tctggaattc ggtctcagga gagcggagaa 541 ttaagggagt cacgttatga cccccgccga tgacgcggga caagccgttt tacgtttgga 601 actgacagaa ccgcaacgtt gaaggagcca ctcagccgcg ggtttctgga gtttaatgag 661 ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag gtcactatca 721 gctagcaaaa atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc 781 atattcactc tcatactcga gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc 841 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac 901 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 961 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 1021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc 1081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 1141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 1201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 1261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 1321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 1381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 1441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 1501 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 1561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 1621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 1681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 1741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 1801 tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg 1861 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa 1921 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact 1981 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct 2041 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa 2101 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc 2161 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt 2221 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt 2281 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atg //