LOCUS Exported 5078 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of Flag tagged human FOXE3 in mammalian cells. ACCESSION . VERSION . KEYWORDS Flag-FOXE3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5078) AUTHORS Moparthi L, Koch S TITLE A uniform expression library for the exploration of FOX transcription factor biology JOURNAL Elsevier 2020 REFERENCE 2 (bases 1 to 5078) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5078 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 35..53 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 35..52 /label=SP6 /note="SP6 promoter, forward primer" regulatory 85..94 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 94..117 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" promoter complement(1108..1125) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 1130..1264 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1154..1173) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1208..1227 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(1377..1397) /label=T3 /note="T3 promoter, forward primer" promoter complement(1377..1395) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(1416..1432) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1416..1432) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1429..1451) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1440..1456 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1464..1494) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1509..1530 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1647..1664) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1818..2406) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1898..1917) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2577..3437) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3200..3219 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3438..3542) /gene="bla" /label=AmpR promoter rep_origin complement(3568..4023) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3705..3726) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3917..3936 /label=F1ori-R /note="F1 origin, reverse primer" promoter 4101..5078 /label=CMV IE94 promoter /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" ORIGIN 1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag 61 ctacttgttc tttttgcagg atcagccacc atggattaca aggacgacga cgataaggga 121 tcccatcgat tcgaattcgc ggggcgcagc gacatggatc cgcccgccgc gttctctggc 181 ttccctgccc tgccagcggt cgcgccgtcg gggccgccgc cgtcgccgct cgcaggagcc 241 gagccagggc gggagccaga ggaggcggcg gctggccgcg gagaggcggc ccccacgccc 301 gcgcccggcc cggggcggcg gcggcggccc ctgcagcgcg ggaagccgcc ctactcgtac 361 atcgcgctca tcgccatggc tctggcgcac gccccgggcc gccgcctcac gctggccgcc 421 atctaccgct tcatcaccga acgctttgcc ttctaccgcg acagcccgcg caagtggcag 481 aacagcatcc gccacaatct cacgctcaac gactgcttcg tcaaggtgcc ccgcgagccg 541 ggcaacccgg gcaagggcaa ctactggacg ctggaccccg cggccgcaga catgttcgac 601 aacggcagct tcctgcggcg ccgcaagcgc ttcaagcgcg ctgagctgcc cgcgcacgcg 661 gccgcggcgc cagggccgcc gctccccttc ccctacgcgc cctacgcgcc cgcgcccggc 721 cccgcgctgc tggtgccgcc gccttctgcc ggaccgggcc cctcgccgcc cgcgcgtctg 781 ttcagcgtcg acagcctggt gaacctgcag ccggagctag cggggctggg cgcccccgag 841 ccgccctgct gcgccgcgcc cgacgccgca gccgcagcct tcccgccctg cgctgccgcc 901 gcctccccgc cactctactc gcaggtcccc gaccgcctgg tactgcccgc gacgcgcccc 961 ggccccggcc cgctgcccgc tgagcccctc ctggccttgg ccgggccggc agccgctctc 1021 ggcccgctca gccctgggga ggcctacctg aggcagccgg gcttcgcgtc ggggctggag 1081 cgctacctgt gactcgagcc tctagaacta tagtgagtcg tattacgtag atccagacat 1141 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt 1201 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca 1261 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt 1321 tttttaattc gcggccgcgg cgccaatgca ttgggcccgg tacccagctt ttgttccctt 1381 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat 1441 tgttatccgc tcacaattcc acacaacata cgagccggga gcataaagtg taaagcctgg 1501 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 1561 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 1621 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 1681 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 1741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 1801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 1861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 1921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 1981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 2041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 2101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 2161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 2221 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 2281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 2341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 2401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 2461 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 2521 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 2581 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 2641 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 2701 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 2761 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 2821 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 2881 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 2941 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 3001 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 3061 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 3121 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 3181 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 3241 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 3301 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 3361 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 3421 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 3481 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 3541 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg 3601 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 3661 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 3721 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 3781 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 3841 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 3901 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 3961 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 4021 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 4081 cgctattacg ccagtcgacc gccaattcaa tatggcgtat atggactcat gccaattcaa 4141 tatggtggat ctggacctgt gccaattcaa tatggcgtat atggactcgt gccaattcaa 4201 tatggtggat ctggacccca gccaattcaa tatggcggac ttggcaccat gccaattcaa 4261 tatggcggac ctggcactgt gccaactggg gaggggtcta cttggcacgg tgccaagttt 4321 gaggaggggt cttggccctg tgccaagtcc gccatattga attggcatgg tgccaataat 4381 ggcggccata ttggctatat gccaggatca atatataggc aatatccaat atggccctat 4441 gccaatatgg ctattggcca ggttcaatac tatgtattgg ccctatgcca tatagtattc 4501 catatatggg ttttcctatt gacgtagata gcccctccca atgggcggtc ccatatacca 4561 tatatggggc ttcctaatac cgcccatagc cactccccca ttgacgtcaa tggtctctat 4621 atatggtctt tcctattgac gtcatatggg cggtcctatt gacgtatatg gcgcctcccc 4681 cattgacgtc aattacggta aatggcccgc ctggctcaat gcccattgac gtcaatagga 4741 ccacccacca ttgacgtcaa tgggatggct cattgcccat tcatatccgt tctcacgccc 4801 cctattgacg tcaatgacgg taaatggccc acttggcagt acatcaatat ctattaatag 4861 taacttggca agtacattac tattggaagt acgccagggt acattggcag tactcccatt 4921 gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt acatccccat tgacgtcaat 4981 ggggaggggc aatgacgcaa atgggcgttc cattgacgta aatgggcggt aggcgtgcct 5041 aatgggaggt ctatataagc aatgctcgtt tagggaac //