LOCUS Exported 2215 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0368 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2215) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2215) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2215 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 195..213 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 217..260 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 451..508 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(532..549) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(703..1291) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(783..802) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1474..1568 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1589..33) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 2163..2182 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" ORIGIN 1 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct 61 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc 121 tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga 181 cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag 241 ctttcgctaa ggatgatttc tggaattcgg tctcaggagg gaggcgtgtc gttttagtga 301 ggatctctct gccgacagtg gtcccaaatc tctctgccga cagtggtccc aaatctctct 361 gccgacagtg gtcccaaact atataaggtt ttgctattca ttgaaagcag tagtgactga 421 tttgtatata atgtactcga gaccctgcag tccggcaaaa aagggcaagg tgtcaccacc 481 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac 541 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 601 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 661 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc 721 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 781 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 841 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 901 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 961 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 1021 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 1081 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 1141 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 1201 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 1261 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 1321 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 1381 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 1441 tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg 1501 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa 1561 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact 1621 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct 1681 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa 1741 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc 1801 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt 1861 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt 1921 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag 1981 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat 2041 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt 2101 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt 2161 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatc //