LOCUS Exported 2209 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0435 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2209) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2209) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2209 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 144..163 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 391..409 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 413..456 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 641..698 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(722..739) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(893..1481) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(973..992) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1664..1758 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1779..229) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 61 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 121 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 181 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 241 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat 301 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa 361 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga 421 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcggtctc aggagggagg 481 cgtgtcgttt tagtgaggtg aagataagat aataatgttg aagataagag tgggagccac 541 cagctttgtc aaaagctaaa aaagatgatg cctatataag gttttgctat tcattgaaag 601 cagtagtgac tgatttgtat atatactcga gaccctgcag tccggcaaaa aagggcaagg 661 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt 721 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 781 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 841 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca 901 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 961 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 1021 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 1081 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 1141 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 1201 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 1261 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 1321 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 1381 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 1441 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 1501 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 1561 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 1621 aaagtatata tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa 1681 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg 1741 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca 1801 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca 1861 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc 1921 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg 1981 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa 2041 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg 2101 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg 2161 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttca //