LOCUS Exported 2213 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0437 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2213) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2213) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2213 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 51..70 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 298..316 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 320..363 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 552..609 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(633..650) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(804..1392) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(884..903) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1575..1669 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1690..136) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact 61 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag 121 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata 181 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca 241 actcgagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 301 aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat 361 ttctggaatt cggtctcagg agggaggcgt gtcgttttag tgaggaagat tgatgaaaag 421 tcaaaaacaa aaatcaatta tgcacaccag catgtgttga tcaccagctc agccacttgt 481 gtctatataa ggttttgcta ttcattgaaa gcagtagtga ctgatttgta tatatactcg 541 agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa 601 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc 661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 781 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa 841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 1021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 1081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 1141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 1201 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 1261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 1321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 1381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 1441 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 1501 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 1561 agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac 1621 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc 1681 gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat 1741 tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag 1801 caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc 1861 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa 1921 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac 1981 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga 2041 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat 2101 caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc 2161 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tct //