LOCUS Exported 2214 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0388 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2214) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2214) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2214 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 118..137 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 365..383 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 387..430 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 620..677 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(701..718) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(872..1460) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(952..971) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1643..1737 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1758..203) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt 61 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca 121 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta 181 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca 241 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc 301 ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 361 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa 421 ggatgatttc tggaattcgg tctcaggagg gaggcgtgtc gttttagtga ggtccatcaa 481 caaataatcc aagtaagtcc atcaacaaat aatccaagta agtccatcaa caaataatcc 541 aagtaagcta tataaggttt tgctattcat tgaaagcagt agtgactgat ttgtatataa 601 tgtactcgag accctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt 661 ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc 721 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 781 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc 901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 1021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 1081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 1141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 1201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 1261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 1321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 1381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 1441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 1501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 1561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 1621 ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg 1681 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa 1741 gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca 1801 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc 1861 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag 1921 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct 1981 gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa 2041 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc 2101 cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta 2161 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agca //