LOCUS Exported 2362 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0313 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2362) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2362) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2362 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 264..283 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 511..529 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 533..576 /label=bacterial terminator /note="putative bacterial transcription terminator" misc_feature 579..600 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" terminator 914..971 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(995..1012) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1166..1754) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1246..1265) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1937..2031 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2052..349) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt tcagtttgct 61 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 121 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 181 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 241 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 301 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 361 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat 421 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa 481 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga 541 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag 601 cgatgagtgg tctcgggagg gaggcgtgtc gttttagtga ggtcattcaa gatctctctg 661 ccgacagtgg tcccaaagat ggaccccaag aggttccaac cacgtctaca aagcaagtgg 721 attgatgtga catctccact gacgtaaggg atgacgcaca atcccactat tttattgggt 781 acccgaataa ctcagcatct tttgctaaat tctatcgtgg ctatataagg ttttgctatt 841 cattgaaagc agtagtgact gatttgtata tatacttgag accactcatc gcgactagta 901 gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa 961 aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg 1021 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 1081 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 1141 gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca 1201 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 1261 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 1321 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 1381 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 1441 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 1501 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 1561 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta 1621 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 1681 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 1741 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 1801 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 1861 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 1921 acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga 1981 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc 2041 tcgatatcaa attacgcccc gccctgccac tcgtcgcagt actgttgtaa ttcattaagc 2101 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc 2161 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg 2221 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgatacg 2281 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc 2341 acatcttgcg aatatatgtg ta //