LOCUS Exported 2268 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0396 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2268) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2268) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2268 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 157..176 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 404..422 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 426..469 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 713..770 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(794..811) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(965..1553) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1045..1064) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1736..1830 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1851..242) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg 61 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta 121 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa 181 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc 241 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat 301 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc 361 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 421 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcggt 481 ctcaggaggg aggcgtgtcg ttttagtgag gggaccgatg ctctctctgc cgacagtggt 541 cccaaacaca tgtaggacat gtaggcaacc acgtctacaa agcaagtgga tgtctgcacc 601 tctctacaaa actgacgtaa gggatgacgc acacttgatg atactatata aggttttgct 661 attcattgaa agcagtagtg actgatttgt atatatactc gagaccctgc agtccggcaa 721 aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg 781 ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 961 cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1381 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 1441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct 1741 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg 1801 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg 1861 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca 1921 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta 1981 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa 2041 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac 2101 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt 2161 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc 2221 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagc //