LOCUS Exported 2339 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0407 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2339) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2339) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2339 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 147..241 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(262..921) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 836..855 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 1083..1101 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 1105..1148 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 1463..1520 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1544..1561) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1715..2303) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1795..1814) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 61 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 121 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg 181 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt 241 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa 301 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc 361 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc 421 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt 481 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc 541 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc 601 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac 661 actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt 721 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac 781 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac 841 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata 901 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa 961 aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc 1021 gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 1081 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg 1141 atgatttctg gaattcggtc tcaggaggga ggcgtgtcgt tttagtgagg cagccacttg 1201 tgttctgcac ctcacatgta ggctatcagc tagcaagtgg ataggttgtc tgcacctcac 1261 atgttctctc tgccgacagt ggtcccaaaa aactggtact tgtgtacagg gctcaaacca 1321 ttattgcgtc gcggtaggtc acgaccacta tgcccaaaaa tgtcaaagat aatcagctta 1381 gcaagacctc tacctatata aggttttgct attcattgaa agcagtagtg actgatttgt 1441 atatatactc gagaccctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt 1501 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc 1561 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 1621 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 1681 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc ccccctgacg 1741 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 1801 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 1861 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 1921 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 1981 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 2041 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 2101 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 2161 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 2221 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 2281 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgct //