LOCUS Exported 2214 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0409 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2214) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2214) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2214 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 438..457 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 685..703 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 707..750 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 940..997 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1021..1038) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1192..1780) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1272..1291) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1963..2057 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2078..523) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac 61 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag 121 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact 181 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga 241 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca 301 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa 361 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct 421 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt 481 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc 541 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa 601 agttggaacc tcttacgtgc ccgatcaact cgagtgccac ctgacgtcta agaaaccatt 661 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa 721 aaatccttag ctttcgctaa ggatgatttc tggaattcgg tctcaggagg gaggcgtgtc 781 gttttagtga ggagcaagtg gatgcccaat taggttgtct gcatgaagat aagataataa 841 tgttgaagat aagagcaaga cctctacaaa ctatataagg ttttgctatt cattgaaagc 901 agtagtgact gatttgtata tatactcgag accctgcagt ccggcaaaaa agggcaaggt 961 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc 1021 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 1081 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 1141 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag 1201 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 1261 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 1321 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 1381 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 1441 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 1501 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 1561 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 1621 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 1681 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 1741 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 1801 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 1861 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 1921 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa 1981 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg 2041 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat 2101 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat 2161 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatt //