LOCUS Exported 2312 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0413 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2312) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2312) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2312 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 21..115 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(136..795) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 710..729 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 957..975 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 979..1022 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 1310..1367 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1391..1408) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1562..2150) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1642..1661) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt 61 ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc 121 gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt 181 aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg 241 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa 301 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga 361 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca 421 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca 481 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc 541 ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag 601 gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt 661 taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg 721 aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt 781 gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac 841 gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa 901 ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 961 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt 1021 tctggaattc ggtctcagga gggaggcgtg tcgttttagt gaggtcagaa gatcaaaggg 1081 ctagctagga ggaatgacgt aagccatgac gtctaccgat gctgatcttg ccaaaaatgt 1141 caaagatacg actgttatgc ccaattaggt tgcaaatatt tcttgtgacc actatgccca 1201 attaggttct gacgtaaggg atgacgcaca ggaggaccga ctatataagg ttttgctatt 1261 cattgaaagc agtagtgact gatttgtata tatactcgag accctgcagt ccggcaaaaa 1321 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta 1381 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 1441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 1501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 1561 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 1621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 1681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 1741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 1801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 1861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 1921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 1981 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 2041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 2101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 2161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 2221 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 2281 aatcaatcta aagtatatat gagtaaactt gg //