LOCUS Exported 2380 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0423 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2380) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2380) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2380 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 254..273 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 501..519 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 523..566 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 922..979 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1003..1020) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1174..1762) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1254..1273) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1945..2039 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2060..339) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac 61 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg 121 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 181 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 241 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 301 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 361 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 421 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta 481 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat 541 ccttagcttt cgctaaggat gatttctgga attcggtctc aggagggagg cgtgtcgttt 601 tagtgaggat tgcgataaag gaaaggccca attaggttgt ctgcacagca agtggatact 661 tgtgtttttc aacaagcggg cacaccagca tgtgttgatc accagcttgc ccaataacca 721 cgtctacaac tctacaaaac tggtacttaa aaatgtcaaa gatagctatc agcttagcca 781 aatatttctt gtgaccgatc agtggtccct ccacggctca cttctctctg ccgacagtgg 841 tcccaaactt agctatataa ggttttgcta ttcattgaaa gcagtagtga ctgatttgta 901 tatatactcg agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt 961 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct 1021 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 1081 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 1141 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga 1201 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 1261 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 1321 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 1381 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 1441 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 1501 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 1561 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 1621 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 1681 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1741 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1801 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1861 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1921 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag 1981 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc 2041 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt 2101 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca 2161 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga 2221 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg 2281 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt 2341 aacacgccac atcttgcgaa tatatgtgta gaaactgccg //