LOCUS Exported 2394 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0427 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2394) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2394) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2394 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 341..360 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 588..606 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 610..653 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 1023..1080 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(1104..1121) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1275..1863) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1355..1374) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2046..2140 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(2161..426) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc 61 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga 121 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac 181 cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca ggcgggcaag 241 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc 301 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc 361 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt 421 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag 481 tgatcttatt tcattatggt gaaagttgga acctcttacg tgcccgatca actcgagtgc 541 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 601 cgaggcagaa tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt 661 cggtctcagg agggaggcgt gtcgttttag tgaggattgc gataaaggaa aggctggtgg 721 aaaaagaaga ggtttgtgtg cacaccagca tgtgttgatc accagctaaa ctggtactta 781 ggtggctcct actgtaggct atcaatcctt accgctatgg gtaagattgg accgatgctg 841 ttatgacccc cgccgatgac gcgggatgtc tgcacctctg gtggagcacg acactctaca 901 aaactggcag ccacttgtgt gcacctctct ctctgccgac agtggtccca aagctatata 961 aggttttgct attcattgaa agcagtagtg actgatttgt atatatactc gagaccctgc 1021 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga 1081 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 1141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 1201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 1261 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc 1321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 1381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 1441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 1501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 1561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 1621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 1681 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg 1741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 1801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 1861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 1921 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 1981 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagctcgagg 2041 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga 2101 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa 2161 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac 2221 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc 2281 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc 2341 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaa //