LOCUS Exported 2279 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0430 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2279) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2279) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2279 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 139..158 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 386..404 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 408..451 /label=bacterial terminator /note="putative bacterial transcription terminator" terminator 706..763 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(787..804) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(958..1546) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1038..1057) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1729..1823 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(1844..224) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" ORIGIN 1 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa 61 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc 121 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat 181 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct 241 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga 301 aagttggaac ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat 361 tattatcatg acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa 421 aaaatcctta gctttcgcta aggatgattt ctggaattcg gtctcaggag ggaggcgtgt 481 cgttttagtg aggtgaagat aagataataa tgttgaagat aagatatcct taccgctatg 541 ggtaagattc atgtaggctt tatgaccccc gccgatgacg cgggagtctc tctgccgaca 601 gtggtcccaa atgctgacgt aagggatgac gcacatctat ataaggtttt gctattcatt 661 gaaagcagta gtgactgatt tgtatatata ctcgagaccc tgcagtccgg caaaaaaggg 721 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca 781 ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 841 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 901 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 961 ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 1021 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 1081 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 1141 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 1201 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 1261 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 1321 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 1381 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 1441 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 1501 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 1561 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 1621 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 1681 aatctaaagt atatatgagt aaacttggtc tgacagctcg aggcttggat tctcaccaat 1741 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat 1801 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc 1861 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa 1921 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt 1981 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac 2041 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag 2101 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact 2161 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga 2221 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcc //