LOCUS Exported 3710 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPAS0CM0008 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3710) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 3710) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3710 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 28..122 /label=lambda t0 terminator /note="transcription terminator from phage lambda" CDS complement(143..802) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 717..736 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" primer_bind 964..982 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" terminator 986..1029 /label=bacterial terminator /note="putative bacterial transcription terminator" CDS 1046..2695 /codon_start=1 /gene="luc+" /product="firefly luciferase" /label=luciferase /note="enhanced luc+ version of the luciferase gene" /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" primer_bind complement(1112..1130) /label=LucNrev /note="Firefly luciferase, reverse primer" terminator 2715..2772 /label=his operon terminator /note="This putative transcriptin terminator from the E. coli his operon has a 2-bp deletion introduced during synthesis. Its efficiency has not been determined." primer_bind complement(2796..2813) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2967..3555) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3047..3066) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 aacttggtct gacagctcga ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc 61 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag 121 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta 181 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg 241 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg 301 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat 361 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac 421 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt 481 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa 541 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat 601 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta 661 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa 721 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat 781 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa 841 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc 901 cgatcaactc gagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 961 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag 1021 gatgatttct ggaattcggt ctcaaatgga ggacgccaaa aacataaaga aaggcccggc 1081 gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag 1141 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac 1201 ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct 1261 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt 1321 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg 1381 tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg 1441 gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat 1501 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct 1561 acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat 1621 tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca 1681 tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat 1741 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac 1801 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagaact 1861 gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt 1921 ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat 1981 tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca 2041 tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac 2101 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa 2161 ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt 2221 gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat 2281 tgacaaggat ggatggctac attctggaga catagcttac tgggacgagg acgaacactt 2341 cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc 2401 tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct 2461 gcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac 2521 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt 2581 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc 2641 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgggttc 2701 gcgagaccct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta 2761 aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg 2821 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 2881 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 2941 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac 3001 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 3061 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 3121 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 3181 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 3241 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 3301 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 3361 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 3421 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 3481 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 3541 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 3601 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 3661 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta //