LOCUS Exported 2760 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pICSL20002 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2760) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2760) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2760 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(36..54) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 122..226 /gene="bla" /label=AmpR promoter CDS 446..1237 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 1408..1996 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1897..1916 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2150..2167 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata 61 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt 121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa 241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc 301 ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg 361 ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag 421 tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca 481 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca 541 tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt 601 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga 661 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt 721 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg 781 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc 841 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga 901 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct 961 atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg 1021 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc 1081 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct 1141 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta 1201 cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata 1261 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 1321 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 1381 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 1441 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 1501 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 1561 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 1621 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 1681 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 1741 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 1801 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 1861 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 1921 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 1981 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2041 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 2101 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 2161 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 2221 tcattaatca ctctgtggtc tcatactgta ttaaaatctt aataggtttt gataaaagcg 2281 aacgtgggga aacccgaacc aaaccttctt ctaaactctc tctcatctct cttaaagcaa 2341 acttctctct tgtctttctt gcgtgagcga tcttcaacgt tgtcagatcg tgcttcggca 2401 ccagtacaac gttttctttc actgaagcga aatcaaagat ctctttgtgg acacgtagtg 2461 cggcgccatt aaataacgtg tacttgtcct attcttgtcg gtgtggtctt gggaaaagaa 2521 agcttgctgg aggctgctgt tcagccccat acattacttg ttacgattct gctgactttc 2581 ggcgggtgca atatctctac ttctgcttga cgaggtattg ttgcctgtac ttctttcttc 2641 ttcttcttgc tgattggttc tataagaaat ctagtatttt ctttgaaaca gagttttccc 2701 gtggttttcg aacttggaga aagattgtta agcttctgta tattctgccc aaattaccat //