LOCUS Exported 2705 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0168 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2705) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2705) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2705 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind 723..743 /label=35S promoter /note="CaMV 35S promoter, forward primer" promoter 732..778 /label=minimal CaMV 35S promoter /note="minimal 35S promoter from cauliflower mosaic virus" primer_bind complement(911..928) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1021..1609) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1101..1120) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1783..2577) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2578..2682) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatt cggtctcagg aggtcaacat ggtggagcac gacactctgg tctactccaa 421 aaatgtcaaa gatacagtct cagaagatca aagggctatt gagacttttc aacaaaggat 481 aatttcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcgaaaggac 541 agtagaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggctatcat 601 tcaagagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg 661 tctacaaagc aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc 721 cactatcctt cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca 781 cgctactcga gaccctctgg gcctcatggg ccttcctttc actgcccgct ttccagtcgg 841 gaaacctgtc gtgccagctg cattaacatg gtcatagctg tttccttgcg tattgggcgc 901 tctccgcttc ctcgctcact gactcgctgc gctcggtcgt tcgggtaaag cctggggtgc 961 ctaatgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 1021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 1081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 1141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 1201 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 1261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 1321 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 1381 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 1441 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac 1501 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 1561 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 1621 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 1681 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 1741 atcaatctaa agtatatatg agtaaacttg gtctgacagt tattagaaaa attcatccag 1801 cagacgataa aacgcaatac gctggctatc cggtgccgca atgccataca gcaccagaaa 1861 acgatccgcc cattcgccgc ccagttcttc cgcaatatca cgggtggcca gcgcaatatc 1921 ctgataacga tccgccacgc ccagacggcc gcaatcaata aagccgctaa aacggccatt 1981 ttccaccata atgttcggca ggcacgcatc accatgggtc accaccagat cttcgccatc 2041 cggcatgctc gctttcagac gcgcaaacag ctctgccggt gccaggccct gatgttcttc 2101 atccagatca tcctgatcca ccaggcccgc ttccatacgg gtacgcgcac gttcaatacg 2161 atgtttcgcc tgatgatcaa acggacaggt cgccgggtcc agggtatgca gacgacgcat 2221 ggcatccgcc ataatgctca ctttttctgc cggcgccaga tggctagaca gcagatcctg 2281 acccggcact tcgcccagca gcagccaatc acggcccgct tcggtcacca catccagcac 2341 cgccgcacac ggaacaccgg tggtggccag ccagctcaga cgcgccgctt catcctgcag 2401 ctcgttcagc gcaccgctca gatcggtttt cacaaacagc accggacgac cctgcgcgct 2461 cagacgaaac accgccgcat cagagcagcc aatggtctgc tgcgcccaat catagccaaa 2521 cagacgttcc acccacgctg ccgggctacc cgcatgcagg ccatcctgtt caatcatact 2581 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 2641 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 2701 gccac //