LOCUS Exported 2740 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0175 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2740) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2740) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2740 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(946..963) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1056..1644) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1136..1155) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1818..2612) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2613..2717) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccgcatt cggtctcagg agtggaagat tcagaaaatc tccatcaaca aataatccaa 421 gtaaggatta atggattgat caacatcctt accgctatgg gtaagattga tgaaaagtca 481 aaaacaaaaa tcaattatgc acaccagcat gtgttgatca ccagctattg tgggacacca 541 atttcgtcca cagacatcaa catcttatcg tcctttgaag ataagataat aatgttgaag 601 ataagagtgg gagccaccac taaaacattg ctttgtcaaa agctaaaaaa gatgatgccc 661 gacagccact tgtgtgaagc atgtgaagcc ggtccctcca ctaagaaaat tagtgaagca 721 tcttccagtg gtccctccac tcacagctca atcagtgagc aacaggacga aggaaatccc 781 acaagaattt ccttatataa ggaacacaaa tcagaaggta ctcgagaccc tctgggcctc 841 atgggccttc ctttcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 901 acatggtcat agctgtttcc ttgcgtattg ggcgctctcc gcttcctcgc tcactgactc 961 gctgcgctcg gtcgttcggg taaagcctgg ggtgcctaat gagcaaaagg ccagcaaaag 1021 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 1081 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 1141 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 1201 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 1261 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 1321 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 1381 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 1441 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 1501 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 1561 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 1621 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 1681 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 1741 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 1801 acttggtctg acagttatta gaaaaattca tccagcagac gataaaacgc aatacgctgg 1861 ctatccggtg ccgcaatgcc atacagcacc agaaaacgat ccgcccattc gccgcccagt 1921 tcttccgcaa tatcacgggt ggccagcgca atatcctgat aacgatccgc cacgcccaga 1981 cggccgcaat caataaagcc gctaaaacgg ccattttcca ccataatgtt cggcaggcac 2041 gcatcaccat gggtcaccac cagatcttcg ccatccggca tgctcgcttt cagacgcgca 2101 aacagctctg ccggtgccag gccctgatgt tcttcatcca gatcatcctg atccaccagg 2161 cccgcttcca tacgggtacg cgcacgttca atacgatgtt tcgcctgatg atcaaacgga 2221 caggtcgccg ggtccagggt atgcagacga cgcatggcat ccgccataat gctcactttt 2281 tctgccggcg ccagatggct agacagcaga tcctgacccg gcacttcgcc cagcagcagc 2341 caatcacggc ccgcttcggt caccacatcc agcaccgccg cacacggaac accggtggtg 2401 gccagccagc tcagacgcgc cgcttcatcc tgcagctcgt tcagcgcacc gctcagatcg 2461 gttttcacaa acagcaccgg acgaccctgc gcgctcagac gaaacaccgc cgcatcagag 2521 cagccaatgg tctgctgcgc ccaatcatag ccaaacagac gttccaccca cgctgccggg 2581 ctacccgcat gcaggccatc ctgttcaatc atactcttcc tttttcaata ttattgaagc 2641 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 2701 caaatagggg ttccgcgcac atttccccga aaagtgccac //