LOCUS Exported 2764 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Phytobricks Level 0 part. ACCESSION . VERSION . KEYWORDS pEPYC0CM0172 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2764) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 2764) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2764 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 278..300 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 292..309 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 293..309 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 320..339 /label=T7 /note="T7 promoter, forward primer" promoter 320..338 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 343..354 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(970..987) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1080..1668) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1160..1179) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1842..2636) /codon_start=1 /product="aminoglycoside phosphotransferase" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" promoter complement(2637..2741) /gene="bla" /label=AmpR promoter ORIGIN 1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt 181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattgaagg aaggccgtca 361 aggccacgtg tcttgtccag agctctcggt ctcaggagtg gaagattcag aaaatctcca 421 tcaacaaata atccaagtaa ggattaatgg attgatcaac atccttaccg ctatgggtaa 481 gattgatgaa aagtcaaaaa caaaaatcaa ttatgcacac cagcatgtgt tgatcaccag 541 ctattgtggg acaccaattt cgtccacaga catcaacatc ttatcgtcct tgtgggagcc 601 accactaaaa cattgctttg tcaaaagcta aaaaagatga tgcccgacag ccacttgtgt 661 gaagcatgtg aagccggtcc ctccactaag aaaattagtg aagcatcttc cagtggtccc 721 tccactcaca gctcaatcag tgagcaacag gacgaaggaa atgacgtaag ccatgacgtc 781 taatcccaca agaatttcct tatataagga acacaaatca gaaggtactc gagaccctgg 841 tacctggagc acaagactgg cctcatgggc cttcctttca ctgcccgctt tccagtcggg 901 aaacctgtcg tgccagctgc attaacatgg tcatagctgt ttccttgcgt attgggcgct 961 ctccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cgggtaaagc ctggggtgcc 1021 taatgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 1081 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 1141 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 1201 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 1261 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 1321 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 1381 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 1441 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 1501 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 1561 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 1621 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 1681 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 1741 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 1801 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt attagaaaaa ttcatccagc 1861 agacgataaa acgcaatacg ctggctatcc ggtgccgcaa tgccatacag caccagaaaa 1921 cgatccgccc attcgccgcc cagttcttcc gcaatatcac gggtggccag cgcaatatcc 1981 tgataacgat ccgccacgcc cagacggccg caatcaataa agccgctaaa acggccattt 2041 tccaccataa tgttcggcag gcacgcatca ccatgggtca ccaccagatc ttcgccatcc 2101 ggcatgctcg ctttcagacg cgcaaacagc tctgccggtg ccaggccctg atgttcttca 2161 tccagatcat cctgatccac caggcccgct tccatacggg tacgcgcacg ttcaatacga 2221 tgtttcgcct gatgatcaaa cggacaggtc gccgggtcca gggtatgcag acgacgcatg 2281 gcatccgcca taatgctcac tttttctgcc ggcgccagat ggctagacag cagatcctga 2341 cccggcactt cgcccagcag cagccaatca cggcccgctt cggtcaccac atccagcacc 2401 gccgcacacg gaacaccggt ggtggccagc cagctcagac gcgccgcttc atcctgcagc 2461 tcgttcagcg caccgctcag atcggttttc acaaacagca ccggacgacc ctgcgcgctc 2521 agacgaaaca ccgccgcatc agagcagcca atggtctgct gcgcccaatc atagccaaac 2581 agacgttcca cccacgctgc cgggctaccc gcatgcaggc catcctgttc aatcatactc 2641 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 2701 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 2761 ccac //