LOCUS Exported 10852 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION GoldenBraid level alpha effector. ACCESSION . VERSION . KEYWORDS pEPYC-alpha-1KN0007 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10852) AUTHORS Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ TITLE Rational design of minimal synthetic promoters for plants. JOURNAL Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682. PUBMED 32856047 REFERENCE 2 (bases 1 to 10852) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10852 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 17..41 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 1342..1971 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIGTHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 2405..3472 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV MRYRNLIEGEASAGS" rep_origin 3538..3732 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(3968..3990) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 4076..4216 /label=bom /note="basis of mobility region from pBR322" primer_bind 4131..4150 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(4231..4248) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4402..4990) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4482..4501) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5078..5893) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 5801..5820 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" misc_feature 6326..6350 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" misc_feature 6663..6718 /label=TMV Omega /note="translational enhancer from the tobacco mosaic virus 5'-leader sequence (Gallie et al., 1988)" terminator 10138..10845 /label=OCS terminator /note="octopine synthase terminator" ORIGIN 1 gtcatgagac gaattctgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt 61 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat 121 gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct 181 gctatagtgc agtcggcttc tgacgttcag tgcagccgtc atctgaaaac gacatgtcgc 241 acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg 301 cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga 361 acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact 421 tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga 481 tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg 541 gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg 601 acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg 661 ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt 721 tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag 781 gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc 841 tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct 901 cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc 961 gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg 1021 aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat 1081 taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca 1141 cctctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc 1201 ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt 1261 atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatccgat gagtaaataa 1321 acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca 1381 ggcaagacga ccatcggaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt 1441 ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat 1501 caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc 1561 ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc 1621 gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg 1681 gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta 1741 caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc 1801 gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc 1861 tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct 1921 gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag 1981 gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca 2041 gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact 2101 ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga 2161 ccattaccga gctgctatct gaatagatcg cgcagctacc agagtaaatg agcaaatgaa 2221 taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca 2281 ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc 2341 ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg 2401 tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt 2461 ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc 2521 cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc 2581 agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt 2641 tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt 2701 ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg 2761 gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt 2821 actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga 2881 caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc 2941 cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca 3001 cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg 3061 tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat 3121 cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt 3181 gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg 3241 cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga 3301 acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg 3361 gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct 3421 agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga 3481 gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt 3541 ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg 3601 gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa 3661 aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct 3721 ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct 3781 tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg 3841 ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc 3901 gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg 3961 gtgaaaacct ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg 4021 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 4081 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 4141 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 4201 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 4261 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 4321 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 4381 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 4441 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 4501 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 4561 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 4621 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 4681 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 4741 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 4801 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 4861 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 4921 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 4981 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5041 ctcacgttaa gggattttgg tcatgcattc taggtgatta gaaaaactca tcgagcatca 5101 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt 5161 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc 5221 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa 5281 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa 5341 gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat 5401 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgagt cgaaatacgc 5461 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg 5521 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg 5581 ttttccctgg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct 5641 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa 5701 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc 5761 catacaatcg gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc 5821 catataaatc agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt 5881 gaatatggct cataacagaa cttattattt ccttcctctt ttctacagta tttaaagata 5941 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac 6001 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta 6061 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac 6121 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg 6181 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc 6241 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc 6301 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac ataacgaatt 6361 cgtctcagga gggaggcgtg tcgttttagt gaggagcaag tggatgtagg tcacgaccat 6421 cactatcagc gatcttgcct gccttttatg acccccgccg atgacgcggg aaggaggacc 6481 gatgctgatt ctctctgccg acagtggtcc caaaggttgt ctgcaccaac cattattgcg 6541 tcacatgtag gctatcagct tatgacgtaa gccatgacgt ctagacctct acaaactata 6601 taaggttttg ctattcattg aaagcagtag tgactgattt gtatatatac tgtattttta 6661 caacaattac caacaacaac aaacaacaaa caacattaca attactattt acaattacaa 6721 tggatcccat tcgcagtcgt acgcctagtc ctgctagaga gcttttacct ggaccacagc 6781 cagatcgagt gcagcctaca gctgatcgtg ggggcgcgcc acccgctggt ggacctctcg 6841 atgggttgcc ggctcgaagg acaatgtcca gaactcgttt accttcacct cctgccccat 6901 cacctgcttt ctccgccggg tcttttaatg accttttgag gcaatttgat ccatcacttc 6961 ttgatacaag tttgttagat agcatgccgg ctgtaggaac tccacacacc gcggcagctc 7021 ctgctgaatg ggacgaagtg cagtccggat tgagagcagc tgatgatcct ccccctacag 7081 ttagagttgc agttacggct gcaagacccc cacgcgcaaa gccagcgcca cgacgtagag 7141 ccgctcagcc cagtgatgct agcccggcag cacaagttga tctcaggact cttggttatt 7201 cacagcaaca gcaggaaaag attaaatcta aagtaaggtc cactgtggct caacatcatg 7261 aggctctcgt aggtcacgga ttcactcatg ctcatatagt tgcattatct cagcatccag 7321 ccgctctcgg taccgtggca gtgaagtacc agcatattat tacagctttg cctgaagcta 7381 cgcatgagga tattgttgga gttggcaagc agtggagcgg tgccagagca ctcgaagcac 7441 ttcttactaa agctggagag ttgagaggtc cgccactaca attggacacc gggcagttgc 7501 ttaaaatcgc taagcggggg ggagttactg ccgttgaggc ggtacacgca tcgagaaatg 7561 cattgactgg agcccccctg aaccttacac cagaccaagt ggtcgctatc gctagtaata 7621 tcggaggcaa tcaggcgtta gagacagtgc aacgtctatt acccgtgcta tgccaggatc 7681 atgggctcac tcccgatcag gtcgtcgcca tcgctaacaa caatggcggt aaacaagcat 7741 tagaaaccgt ccaaaggttg ctgccagtcc tctgtcaggc tcacggcctt acacctgatc 7801 aagttgtagc tattgcatct aacggaggca agcaggcact tgagactgtc cagaggcttc 7861 tgccagtttt atgccaggct catggtctga cccctaatca ggtggttgcc atcgcgtcta 7921 atggtggggg aaaacaagct ctcgaaactg ttcagaggtt attaccagtc ctttgtcaag 7981 ctcatggcct tactcaagat caagtagttg cgatcgccag taactctgga ggtaagcaag 8041 cccttgaaac cgtgcaaagg ctcctccctg tactctgcca ggcgcatggc ctcacgcctg 8101 ctcaggtggt agctatcgca tctaacaatg gtggtaagca ggcattggaa acagtccaga 8161 gactcttccc agtcctgtgc caagatcatg ggttgacccc cgatcaagtg gtgactatag 8221 caaataacaa tggagggaag caggcgctgg agacagttca acgactcctt cctgttctct 8281 gtcaagcaca cggtttgatt ccagatcagg tagtggccat tgccaacaat aacgggggca 8341 agcaagcgtt agaaactgta caacggcttc ttccagtgct atgtcaagcc cacgggttga 8401 ctccagcaca ggttgtcgct attgcttcaa acataggcgg aaagcaagca ctggaaaccg 8461 tgcagcgact tctaccagta ctatgtcgag ctcacggact tacccctgct caagttgtcg 8521 caattgccaa taataatggt ggcaaacagg ctcttgaaac agttcaaagg cttctccctg 8581 tcctatgtca ggcacatggc ctgacacccg accaagtcgt tgccatagcc agcaatattg 8641 gtgggaagca agcattggag actgtgcaac ggttattgcc tgtcctttgc caagatcatg 8701 gtttgacgcc tgaccaagtt gtggcgattg cctctaatgg aggaaagcag gcccttgaga 8761 cagtccagcg gctactccct gtcttatgcc aagaccatgg cttgacccct gcacaagtgg 8821 ttgcgatagc atcacatgat gggggtaaac aggctttgga aacggttcag aggctactgc 8881 cagtattgtg ccaggaccat ggcctaacac tggatcaagt tgttgctata gcgtcgcacg 8941 atggaggcaa acaagcgttg gaaaccgttc aaagattgct gcctgttttg tgtcaagatc 9001 acggtcttac actagatcag gttgtggcaa tcgcttctaa tatcgggggt aagcaggcct 9061 tagagacagt acaaagatta ctaccagttc tgtgtcagga tcacggactg actcctgacc 9121 aggtagttgc tatcgcttca aatggtggcg ggaaacaggc gcttgagaca gtacagaggt 9181 tgcttccagt tctttgccag gatcatggac ttactccaga ccaggtggtg gctattgcta 9241 gtaatggcgg tggaaaacag gccttggaat caattgttgc tcagttgtca agacctgatc 9301 ctgccctggc tgccctaact aatgaccacc ttgtggcctt agcgtgtctt ggtgggcgtc 9361 cggcattaga tgccgtaaaa aagggccttc cacacgcccc tgagttgata cgtagaatta 9421 atcgtcgcat tcctgaacgc acctctcatc gtgtggcaga ccttgcgcat gttgttagag 9481 ttttaggttt ctttcaaagt cactcccatc ctgcccaggc tttcgatgat gctatgaccc 9541 aatttggaat gagtcggcat ggcttggtcc agctatttag aagggttggt gttactgagt 9601 ttgaggctcg ttgtggcaca ctgccgccag cctcacaaag atgggacagg attttgcaag 9661 cgtctggtat gaagcgtgca aagccatccc ctaccagcgc tcaaacaccg gatcaagcct 9721 ctttacatgc ctttgctgac tcccttgaga gagatttaga tgctccatcc ccgatgcatg 9781 aaggcgacca gactagagct tcatcgagga agagatcccg aagtgatcga gcagtaactg 9841 gtccttcgac acagcagtct ttcgaggtta gagtcccaga acaacgcgat gctctacact 9901 tacccttgtc ttggagagtc aaacgcccac gtactaggat aggaggcggt ttaccagatc 9961 cgggaacacc tatcgcagca gacttggctg catctagtac tgttatgtgg gaacaggacg 10021 ctgctccttt tgctggtgca gctgacgatt tccctgcttt taacgaagaa gaattggcat 10081 ggctcatgga gctgcttcca caatcgggta gtgtcggggg tacgatctga gcttgtcctg 10141 ctttaatgag atatgcgaga agcctatgat cgcatgatat ttgctttcaa ttctgttgtg 10201 cacgttgtaa aaaacctgag catgtgtagc tcagatcctt accgccggtt tcggttcatt 10261 ctaatgaata tatcacccgt tactatcgta tttttatgaa taatattctc cgttcaattt 10321 actgattgta ccctactact tatatgtaca atattaaaat gaaaacaata tattgtgctg 10381 aataggttta tagcgacatc tatgatagag cgccacaata acaaacaatt gcgttttatt 10441 attacaaatc caattttaaa aaaagcggca gaaccggtca aacctaaaag actgattaca 10501 taaatcttat tcaaatttca aaagtgcccc aggggctagt atctacgaca caccgagcgg 10561 cgaactaata acgctcactg aagggaactc cggttccccg ccggcgcgca tgggtgagat 10621 tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc attcaacccg 10681 gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag catttttttg 10741 gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac aaatcgttgg 10801 gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggaccg ct //