LOCUS Exported 5570 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: MT End Binding Protein, Excitation: 433, Emission: 475. ACCESSION . VERSION . KEYWORDS mCerulean3-EB3-7 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5570) AUTHORS Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, Day RN, Wachter RM, Davidson MW, Rizzo MA TITLE An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching. JOURNAL PLoS One. 2011 . 6(3):e17896. PUBMED 21479270 REFERENCE 2 (bases 1 to 5570) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5570 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 44..347 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 348..551 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 502..522 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 629..638 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 1493..1502 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(1544..1565) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1805..1824) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 2152..2173 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 2341..2462 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2378..2397) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2432..2451 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2469..2924) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2606..2627) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2818..2837 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2951..3055 /gene="bla" /label=AmpR promoter promoter 3057..3414 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3080..3100) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3265..3400 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3327..3346 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3449..4243 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3503..3522) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4113..4132 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4431..4450) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4475..4522 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4851..5439 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5340..5359 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 61 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 121 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 181 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 241 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 301 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 361 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 421 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 481 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 541 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga 601 gctcaagctt cgaattctgc agtcgactgc caccatggcc gtcaatgtgt actccacatc 661 tgtgaccagt gaaaatctga gtcgccatga tatgcttgca tgggtcaacg actccctgca 721 cctcaactat accaagatag aacagctttg ttcaggggca gcctactgcc agttcatgga 781 catgctcttc cccggctgtg tgcacttgag gaaagtgaag ttccaggcca aactagagca 841 tgaatacatc cacaacttca aggtgctgca agcagctttc aagaagatgg gtgttgacaa 901 aatcattcct gtagagaaat tagtgaaagg aaaattccaa gataattttg agtttattca 961 gtggtttaag aaattctttg acgcaaacta tgatggaaag gattacaacc ctctgctggc 1021 gcggcagggc caggacgtag cgccacctcc taacccaggt gatcagatct tcaacaaatc 1081 caagaaactc attggcacag cagttccaca gaggacgtcc cccacaggcc caaaaaacat 1141 gcagacctct ggccggctga gcaatgtggc ccccccctgc attctccgga agaatcctcc 1201 atcagcccga aatggcggcc atgagactga tgcccaaatt cttgaactca accaacagct 1261 ggtggacttg aagctgacag tggatgggct ggagaaggaa cgtgacttct acttcagcaa 1321 acttcgtgac atcgagctca tctgccagga gcatgaaagt gaaaacagcc ctgttatctc 1381 aggcatcatt ggcatcctct atgccacaga ggaaggattc gcaccccctg aggacgatga 1441 gattgaagag catcaacaag aagaccagga cgagtacctg gatccaccgg tcgccaccat 1501 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg 1561 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg 1621 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct 1681 cgtgaccacc ctgagctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca 1741 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt 1801 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt 1861 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa 1921 gctggagtac aacgccatcc acggcaacgt ctatatcacc gccgacaagc agaagaacgg 1981 catcaaggcc aacttcggcc tcaactgcaa catcgaggac ggcagcgtgc agctcgccga 2041 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta 2101 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct 2161 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag 2221 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta 2281 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 2341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 2401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 2461 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat 2521 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata 2581 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt 2641 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc 2701 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa 2761 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg 2821 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt 2881 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg 2941 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 3001 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga 3061 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 3121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag 3181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 3241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 3301 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 3361 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga 3421 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc 3481 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg 3541 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac 3601 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc 3661 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg 3721 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga 3781 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg 3841 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg 3901 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt 3961 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc 4021 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg 4081 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga 4141 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc 4201 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc 4261 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc 4321 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag 4381 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg 4441 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg 4501 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc 4561 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc 4621 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct 4681 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt 4741 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 4801 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 4861 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 4921 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 4981 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct 5041 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 5101 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 5161 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 5221 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 5281 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 5341 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 5401 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 5461 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 5521 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat //