LOCUS Exported 786 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli. ACCESSION . VERSION . KEYWORDS pET28-6His-SUMO-Nsp10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 786) TITLE Plasmids for the Expression and Co-Expression of COVID-19 Proteins Nsp10 and Nsp14 JOURNAL Unpublished REFERENCE 2 (bases 1 to 786) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..786 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 13..30 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 40..57 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" CDS 70..360 /codon_start=1 /gene="S. cerevisiae SMT3 (truncated)" /product="cleavable ubiquitin-like protein tag" /label=SUMO /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG" ORIGIN 1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat 61 atggctagca tgtcggactc agaagtcaat caagaagcta agccagaggt caagccagaa 121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc 181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa aagacagggt 241 aaggaaatgg actccttaag attcttgtac gacggtatta gaattcaagc tgatcagacc 301 cctgaagatt tggacatgga ggataacgat attattgagg ctcacagaga acagattggt 361 ggatccgctg gtaatgcaac tgaggttccc gcgaatagta cggttctgag cttttgcgcc 421 tttgctgttg acgcagctaa ggcatataag gactacttgg cctccggggg ccagccaatc 481 acaaactgcg tcaagatgtt gtgtacgcac acgggcacag gtcaggcaat cactgtcaca 541 cctgaagcta acatggatca ggaatctttt ggtggtgcca gttgttgtct gtactgtcga 601 tgtcatatag atcatcccaa cccgaaaggg ttttgtgatc tgaaaggaaa atacgtccaa 661 atcccgacca cgtgcgcgaa cgacccggtt gggtttacgt tgaaaaatac cgtatgtact 721 gtctgtggta tgtggaaggg ctatggctgt tcatgcgatc aattgcgaga gccgatgttg 781 cagtaa //