LOCUS       Exported                 468 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease 
            cleavable Strep tag in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    pET22-Strep-TEV-Nsp10
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 468)
  TITLE     Plasmids for the Expression and Co-Expression of COVID-19 Proteins 
            Nsp10 and Nsp14
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             4..27
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             28..48
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtggtcac acccccaatt tgaaaaggaa aatctatact tccaaggagc tggtaatgca
       61 actgaggttc ccgcgaatag tacggttctg agcttttgcg cctttgctgt tgacgcagct
      121 aaggcatata aggactactt ggcctccggg ggccagccaa tcacaaactg cgtcaagatg
      181 ttgtgtacgc acacgggcac aggtcaggca atcactgtca cacctgaagc taacatggat
      241 caggaatctt ttggtggtgc cagttgttgt ctgtactgtc gatgtcatat agatcatccc
      301 aacccgaaag ggttttgtga tctgaaagga aaatacgtcc aaatcccgac cacgtgcgcg
      361 aacgacccgg ttgggtttac gttgaaaaat accgtatgta ctgtctgtgg tatgtggaag
      421 ggctatggct gttcatgcga tcaattgcga gagccgatgt tgcagtaa
//
