LOCUS       Exported                3681 bp ds-DNA     linear   SYN 28-MAY-2021
DEFINITION  Lentiviral vector expressing human codon-optimized _C-terminal 
            Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- 
            and C-terminal NLS and Neomycin/G418/Geneticin resistance from 
            EF-1alpha promoter.
ACCESSION   .
VERSION     .
KEYWORDS    plenti-Lb-Cas12a-2xNLS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Gonatopoulos-Pournatzis T, Aregger M, Brown KR, Farhangmehr S, 
            Braunschweig U, Ward HN, Ha KCH, Weiss A, Billmann M, Durbic T, 
            Myers CL, Blencowe BJ, Moffat J
  TITLE     Genetic interaction mapping and exon-resolution functional genomics 
            with a hybrid Cas9-Cas12a platform.
  JOURNAL   Nat Biotechnol. 2020 May;38(5):638-648. doi: 
            10.1038/s41587-020-0437-z. Epub 2020 Mar 16.
  PUBMED    32249828
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..3681
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED
                     YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI
                     AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE
                     EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE
                     FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS
                     DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG
                     PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL
                     QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL
                     LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK
                     DKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN
                     YEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI
                     DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE
                     EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE
                     ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN
                     TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY
                     HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF
                     KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN
                     GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY
                     KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ
                     GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY
                     EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV
                     KH"
ORIGIN
        1 agcaagctgg agaagtttac aaactgctac tccctgtcta agaccctgag gttcaaggcc
       61 atccctgtgg gcaagaccca ggagaacatc gacaataagc ggctgctggt ggaggacgag
      121 aagagagccg aggattataa gggcgtgaag aagctgctgg atcgctacta tctgtctttt
      181 atcaacgacg tgctgcacag catcaagctg aagaatctga acaattacat cagcctgttc
      241 cggaagaaaa ccagaaccga gaaggagaat aaggagctgg agaacctgga gatcaatctg
      301 cggaaggaga tcgccaaggc cttcaagggc aacgagggct acaagtccct gtttaagaag
      361 gatatcatcg agacaatcct gccagagttc ctggacgata aggacgagat cgccctggtg
      421 aacagcttca atggctttac cacagccttc accggcttct ttgataacag agagaatatg
      481 ttttccgagg aggccaagag cacatccatc gccttcaggt gtatcaacga gaatctgacc
      541 cgctacatct ctaatatgga catcttcgag aaggtggacg ccatctttga taagcacgag
      601 gtgcaggaga tcaaggagaa gatcctgaac agcgactatg atgtggagga tttctttgag
      661 ggcgagttct ttaactttgt gctgacacag gagggcatcg acgtgtataa cgccatcatc
      721 ggcggcttcg tgaccgagag cggcgagaag atcaagggcc tgaacgagta catcaacctg
      781 tataatcaga aaaccaagca gaagctgcct aagtttaagc cactgtataa gcaggtgctg
      841 agcgatcggg agtctctgag cttctacggc gagggctata catccgatga ggaggtgctg
      901 gaggtgttta gaaacaccct gaacaagaac agcgagatct tcagctccat caagaagctg
      961 gagaagctgt tcaagaattt tgacgagtac tctagcgccg gcatctttgt gaagaacggc
     1021 cccgccatca gcacaatctc caaggatatc ttcggcgagt ggaacgtgat ccgggacaag
     1081 tggaatgccg agtatgacga tatccacctg aagaagaagg ccgtggtgac cgagaagtac
     1141 gaggacgatc ggagaaagtc cttcaagaag atcggctcct tttctctgga gcagctgcag
     1201 gagtacgccg acgccgatct gtctgtggtg gagaagctga aggagatcat catccagaag
     1261 gtggatgaga tctacaaggt gtatggctcc tctgagaagc tgttcgacgc cgattttgtg
     1321 ctggagaaga gcctgaagaa gaacgacgcc gtggtggcca tcatgaagga cctgctggat
     1381 tctgtgaaga gcttcgagaa ttacatcaag gccttctttg gcgagggcaa ggagacaaac
     1441 agggacgagt ccttctatgg cgattttgtg ctggcctacg acatcctgct gaaggtggac
     1501 cacatctacg atgccatccg caattatgtg acccagaagc cctactctaa ggataagttc
     1561 aagctgtatt ttcagaaccc tcagttcatg ggcggctggg acaaggataa ggagacagac
     1621 tatcgggcca ccatcctgag atacggctcc aagtactatc tggccatcat ggataagaag
     1681 tacgccaagt gcctgcagaa gatcgacaag gacgatgtga acggcaatta cgagaagatc
     1741 aactataagc tgctgcccgg ccctaataag atgctgccaa aggtgttctt ttctaagaag
     1801 tggatggcct actataaccc cagcgaggac atccagaaga tctacaagaa tggcacattc
     1861 aagaagggcg atatgtttaa cctgaatgac tgtcacaagc tgatcgactt ctttaaggat
     1921 agcatctccc ggtatccaaa gtggtccaat gcctacgatt tcaacttttc tgagacagag
     1981 aagtataagg acatcgccgg cttttacaga gaggtggagg agcagggcta taaggtgagc
     2041 ttcgagtctg ccagcaagaa ggaggtggat aagctggtgg aggagggcaa gctgtatatg
     2101 ttccagatct ataacaagga cttttccgat aagtctcacg gcacacccaa tctgcacacc
     2161 atgtacttca agctgctgtt tgacgagaac aatcacggac agatcaggct gagcggagga
     2221 gcagagctgt tcatgaggcg cgcctccctg aagaaggagg agctggtggt gcacccagcc
     2281 aactccccta tcgccaacaa gaatccagat aatcccaaga aaaccacaac cctgtcctac
     2341 gacgtgtata aggataagag gttttctgag gaccagtacg agctgcacat cccaatcgcc
     2401 atcaataagt gccccaagaa catcttcaag atcaatacag aggtgcgcgt gctgctgaag
     2461 cacgacgata acccctatgt gatcggcatc gataggggcg agcgcaatct gctgtatatc
     2521 gtggtggtgg acggcaaggg caacatcgtg gagcagtatt ccctgaacga gatcatcaac
     2581 aacttcaacg gcatcaggat caagacagat taccactctc tgctggacaa gaaggagaag
     2641 gagaggttcg aggcccgcca gaactggacc tccatcgaga atatcaagga gctgaaggcc
     2701 ggctatatct ctcaggtggt gcacaagatc tgcgagctgg tggagaagta cgatgccgtg
     2761 atcgccctgg aggacctgaa ctctggcttt aagaatagcc gcgtgaaggt ggagaagcag
     2821 gtgtatcaga agttcgagaa gatgctgatc gataagctga actacatggt ggacaagaag
     2881 tctaatcctt gtgcaacagg cggcgccctg aagggctatc agatcaccaa taagttcgag
     2941 agctttaagt ccatgtctac ccagaacggc ttcatctttt acatccctgc ctggctgaca
     3001 tccaagatcg atccatctac cggctttgtg aacctgctga aaaccaagta taccagcatc
     3061 gccgattcca agaagttcat cagctccttt gacaggatca tgtacgtgcc cgaggaggat
     3121 ctgttcgagt ttgccctgga ctataagaac ttctctcgca cagacgccga ttacatcaag
     3181 aagtggaagc tgtactccta cggcaaccgg atcagaatct tccggaatcc taagaagaac
     3241 aacgtgttcg actgggagga ggtgtgcctg accagcgcct ataaggagct gttcaacaag
     3301 tacggcatca attatcagca gggcgatatc agagccctgc tgtgcgagca gtccgacaag
     3361 gccttctact ctagctttat ggccctgatg agcctgatgc tgcagatgcg gaacagcatc
     3421 acaggccgca ccgacgtgga ttttctgatc agccctgtga agaactccga cggcatcttc
     3481 tacgatagcc ggaactatga ggcccaggag aatgccatcc tgccaaagaa cgccgacgcc
     3541 aatggcgcct ataacatcgc cagaaaggtg ctgtgggcca tcggccagtt caagaaggcc
     3601 gaggacgaga agctggataa ggtgaagatc gccatctcta acaaggagtg gctggagtac
     3661 gcccagacca gcgtgaagca c
//
