LOCUS Exported 10602 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Express NSP5 gene along with HA and BASU. ACCESSION . VERSION . KEYWORDS SARS_COV2_nsp5_HA_BASU SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10602) TITLE Khavari lab SARS-CoV-2 plasmids REFERENCE 2 (bases 1 to 10602) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10602 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2338..3519 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2568..3510 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3467..3487 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 4489..4512 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 4516..4542 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" misc_feature 5352..5925 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5519..5536) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 5746..5765 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 5958..6557 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5958..5977) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6454..6474 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6571..7159 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6624..6644) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7042..7053) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7366..7999 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8127..8143) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8127..8143) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8140..8162) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8151..8167 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8175..8205) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8220..8241 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8358..8375) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8529..9117) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8609..8628) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9288..10148) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9911..9930 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10149..10253) /gene="bla" /label=AmpR promoter polyA_signal 10301..10435 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10338..10357) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10392..10411 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 3541 gccgccacca tgtctggttt taggaaaatg gcgttcccca gcggtaaagt tgaaggatgt 3601 atggtccaag taacctgtgg taccactacc cttaatgggc tttggttgga cgacgtagtc 3661 tactgccccc gacacgtaat ctgcactagt gaggatatgc ttaatcccaa ttacgaagac 3721 cttttgattc ggaaatccaa tcacaacttc ctggtccaag cgggcaacgt ccaactcagg 3781 gttattggac atagtatgca gaattgcgta ctgaagctca aagtcgatac tgcaaacccc 3841 aagacgccca agtataaatt cgtccgaatc caaccaggcc aaacattttc cgtattggct 3901 tgctataatg gaagccccag cggtgtctac caatgtgcaa tgagaccaaa ctttacgata 3961 aagggttcat ttctgaacgg ctcttgcggt tccgttggtt ttaacatcga ctatgactgt 4021 gtatcctttt gctacatgca ccatatggaa ctccctaccg gtgtccacgc cggtacagat 4081 ctggaaggaa atttctacgg tccgttcgtt gaccggcaaa ccgcgcaagc ggctggaacc 4141 gacacaacga ttacagtgaa tgtgctcgcg tggctgtacg cagcagtcat aaacggagac 4201 aggtggtttc tgaaccgatt tacgacgact ctcaatgact tcaaccttgt tgcgatgaag 4261 tacaattacg agccactcac ccaggaccat gttgatatcc tgggtcccct cagtgcccag 4321 acagggatcg cagttctcga tatgtgcgcg tcactgaagg agcttctcca aaatggaatg 4381 aatgggcgga ccatacttgg ttccgcactc ctcgaagatg aatttactcc atttgacgtg 4441 gtcagacaat gcagtggggt cactttccag ctcgaaggcg gcgggggatg gagccatcca 4501 caattcgaga aaggctaccc atacgatgtg cctgactatg ctggcaagtt gtctgaatca 4561 gagattaggt ttggactgaa aacggaggtg atgggccaac atcttatcta tcacgatgtt 4621 ttgtccagca cacaaaagac agcccatgaa ttggcaaata acaatgcccc cgagggtacc 4681 ctcgtggttg cagataaaca gacagccggt aggggcggga tgagccgagt gtggcattct 4741 caagaaggaa acggagtatg gatgagtttg atattgcgcc cggacattcc gctccaaaag 4801 acgccccaac ttaccttgct ggcagctgta gcggtggttc agggaataga agaggccgcg 4861 ggcatacaga ctgatataaa gtggccgaac gacatactca ttaacgggaa aaaaacagta 4921 gggatactta ccgaaatgca agctgaggag gatcgagtgc gctccgtgat tattggtatt 4981 ggcattaatg taaatcagca acccaatgat ttccccgatg agctcaagga cattgcgacg 5041 agtctgtccc aggcagctgg ggaaaagatc gacagggcag gtgtaatcca gcatatcttg 5101 ctttgtttcg aaaagcgata ccgagactac atgacccacg gtttcacgcc catcaagctc 5161 ttgtgggagt catacgccct cggtattggc acgaacatga gggcgagaac tctcaacggg 5221 acattttacg gtaaggcgtt gggtattgac gacgaagggg tacttttgtt ggaaactaat 5281 gaaggcatta agaagatata cagcgcggac atcagcctta ggtgaccgca gtttgaaaag 5341 taaggatccc gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa 5401 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat 5461 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct 5521 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct 5581 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc 5641 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa 5701 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc 5761 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg 5821 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc 5881 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca 5941 caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 6001 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 6061 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 6121 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 6181 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 6241 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 6301 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 6361 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 6421 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 6481 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 6541 gcaagcccgg tgcctagacg cgtctggaac aatcaacctc tggattacaa aatttgtgaa 6601 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 6661 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 6721 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 6781 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 6841 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 6901 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 6961 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg 7021 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 7081 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 7141 ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag 7201 caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag 7261 aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 7321 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc 7381 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 7441 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 7501 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 7561 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 7621 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 7681 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 7741 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 7801 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 7861 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 7921 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 7981 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 8041 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt accgtcgacc 8101 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 8161 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa 8221 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 8281 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 8341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 8401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 8461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 8521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 8581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 8641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 8701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 8761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 8821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 8881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 8941 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag 9001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 9061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 9121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 9181 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 9241 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 9301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 9361 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 9421 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 9481 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 9541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 9601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 9661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 9721 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 9781 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 9841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 9901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 9961 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 10021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 10081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 10141 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 10201 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 10261 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta ttgcagctta 10321 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 10381 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg 10441 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc 10501 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta 10561 aagaaattgt atttgttaaa tatgtactac aaacttagta gt //