LOCUS Exported 10938 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Express N gene along with HA and BASU. ACCESSION . VERSION . KEYWORDS SARS_COV2_N_HA_BASU SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10938) TITLE Khavari lab SARS-CoV-2 plasmids REFERENCE 2 (bases 1 to 10938) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10938 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2338..3519 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2568..3510 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3467..3487 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 4825..4848 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" CDS 4852..4878 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" misc_feature 5688..6261 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(5855..5872) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6082..6101 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6294..6893 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6294..6313) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6790..6810 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6907..7495 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6960..6980) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7378..7389) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 7702..8335 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8463..8479) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8463..8479) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8476..8498) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8487..8503 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8511..8541) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8556..8577 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8694..8711) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8865..9453) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8945..8964) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9624..10484) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10247..10266 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10485..10589) /gene="bla" /label=AmpR promoter polyA_signal 10637..10771 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10674..10693) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10728..10747 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 3541 gccgccacca tgagcgataa cggcccccaa aaccaacgga atgcccctcg gataacattt 3601 ggtggcccta gtgattccac aggaagtaac caaaacggag aacggtctgg agcccgctct 3661 aaacagaggc gaccacaggg attgccaaat aacacagcgt catggtttac agccctgact 3721 caacatggaa aggaggactt gaaatttcca cgaggacagg gtgtaccaat aaacacaaac 3781 agtagtccag acgatcagat cgggtattat agaagggcca cgagacgcat cagaggtgga 3841 gatggtaaaa tgaaagatct cagtccgcgc tggtactttt attatttggg aaccggccca 3901 gaagcgggcc tcccatatgg agccaataag gacgggatta tatgggtggc tacagaggga 3961 gccctcaata cgccaaaaga tcacatcgga acaagaaacc ctgctaataa tgccgcaatc 4021 gtgctgcagt tgcctcaggg gacgacgctt cctaaaggct tttacgcaga aggatcacgc 4081 ggcggcagcc aagcatcctc aaggtctagt tccagaagtc gaaacagctc cagaaactcc 4141 acaccagggt ccagtagggg cacaagtccg gcgcggatgg cgggcaacgg cggagacgcc 4201 gcactcgctc tgttgctcct ggaccggctc aaccaacttg aatccaagat gagtggtaag 4261 ggacagcagc aacaaggtca aaccgtaacc aagaaaagcg ctgcagaagc ttctaaaaaa 4321 cctcgacaaa aacggaccgc tacgaaggca tataacgtta ctcaagcctt cgggagacga 4381 gggccggagc aaacccaggg gaattttgga gatcaggagc tcatccggca agggacagat 4441 tacaaacatt ggcctcaaat cgctcagttc gcgccgtccg caagtgcttt tttcggaatg 4501 tctcgcatcg gtatggaagt aacaccatcc gggacatggc tcacttatac cggcgccatc 4561 aagctggacg acaaagatcc aaatttcaaa gatcaggtga ttctcttgaa taaacacata 4621 gacgcgtata agacgtttcc acccaccgaa cccaagaagg acaaaaagaa aaaagctgat 4681 gaaacacaag cgttgcccca gcgacagaag aagcagcaaa ccgtcaccct cctgccagcc 4741 gctgatctcg atgacttctc caagcaactt caacagtcca tgagctcagc cgattctact 4801 caggcgctcg aaggcggcgg gggatggagc catccacaat tcgagaaagg ctacccatac 4861 gatgtgcctg actatgctgg caagttgtct gaatcagaga ttaggtttgg actgaaaacg 4921 gaggtgatgg gccaacatct tatctatcac gatgttttgt ccagcacaca aaagacagcc 4981 catgaattgg caaataacaa tgcccccgag ggtaccctcg tggttgcaga taaacagaca 5041 gccggtaggg gcgggatgag ccgagtgtgg cattctcaag aaggaaacgg agtatggatg 5101 agtttgatat tgcgcccgga cattccgctc caaaagacgc cccaacttac cttgctggca 5161 gctgtagcgg tggttcaggg aatagaagag gccgcgggca tacagactga tataaagtgg 5221 ccgaacgaca tactcattaa cgggaaaaaa acagtaggga tacttaccga aatgcaagct 5281 gaggaggatc gagtgcgctc cgtgattatt ggtattggca ttaatgtaaa tcagcaaccc 5341 aatgatttcc ccgatgagct caaggacatt gcgacgagtc tgtcccaggc agctggggaa 5401 aagatcgaca gggcaggtgt aatccagcat atcttgcttt gtttcgaaaa gcgataccga 5461 gactacatga cccacggttt cacgcccatc aagctcttgt gggagtcata cgccctcggt 5521 attggcacga acatgagggc gagaactctc aacgggacat tttacggtaa ggcgttgggt 5581 attgacgacg aaggggtact tttgttggaa actaatgaag gcattaagaa gatatacagc 5641 gcggacatca gccttaggtg accgcagttt gaaaagtaag gatcccgccc ctctccctcc 5701 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat 5761 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct 5821 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg 5881 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta 5941 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag 6001 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg 6061 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat 6121 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca 6181 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc 6241 ctttgaaaaa cacgatgata agcttgccac aacccacaag gagacgacct tccatgaccg 6301 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc 6361 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgacccg gaccgccaca 6421 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 6481 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 6541 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 6601 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 6661 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg 6721 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 6781 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 6841 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tagacgcgtc 6901 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 6961 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 7021 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 7081 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 7141 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 7201 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 7261 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg 7321 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 7381 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 7441 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga 7501 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag 7561 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag 7621 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact 7681 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc 7741 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag 7801 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc 7861 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc 7921 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag 7981 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag 8041 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg 8101 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct 8161 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 8221 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 8281 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt 8341 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt 8401 gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca 8461 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 8521 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 8581 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 8641 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 8701 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 8761 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 8821 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 8881 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 8941 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 9001 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 9061 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 9121 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 9181 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 9241 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 9301 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 9361 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 9421 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 9481 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 9541 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 9601 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 9661 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 9721 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 9781 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 9841 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 9901 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 9961 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 10021 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 10081 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 10141 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 10201 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 10261 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 10321 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 10381 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 10441 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 10501 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 10561 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 10621 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag 10681 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 10741 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca 10801 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact 10861 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg 10921 tactacaaac ttagtagt //