LOCUS Exported 10173 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentiviral vector to generate hACE2 stable expressing cell line. ACCESSION . VERSION . KEYWORDS pLENTI_hACE2_PURO SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10173) TITLE De Francesco Lab Plasmids REFERENCE 2 (bases 1 to 10173) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10173 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 113..134 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 149..179 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 187..203 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 192..214 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 211..227 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 211..227 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 246..266 /label=T3 /note="T3 promoter, forward primer" promoter 248..266 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 294..520 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 521..701 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 748..873 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1366..1599 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1784..1828 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2095..2211 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 2234..2537 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2538..2741 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2692..2712 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2738..2762 /label=LNCX /note="Human CMV promoter, forward primer" primer_bind complement(5300..5320) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5305..5634 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5485..5620 /label=SV40 ori /note="SV40 origin of replication" CDS 5644..6243 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5644..5663) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6140..6160 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 6310..6898 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(6363..6383) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6781..6792) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6985..7218 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 7290..7411 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7327..7346) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7381..7400 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 7451..7586 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(7606..7625) /label=T7 /note="T7 promoter, forward primer" promoter complement(7607..7625) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7635..7652) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7635..7651) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7644..7666) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7793..8248 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7880..7899) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 8090..8111 /label=F1ori-F /note="F1 origin, forward primer" promoter 8274..8378 /gene="bla" /label=AmpR promoter CDS 8379..9239 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8597..8616) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9410..9998 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9899..9918 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10152..10169 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 61 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 121 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 181 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 241 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc 301 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca 361 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt 421 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg 481 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca 541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac 721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg 781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga 841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg 901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata 961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca 1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa 1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata 1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc 1201 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag 1261 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa 1321 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct 1381 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca 1441 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga 1501 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat 1561 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg 1621 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga 1681 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag 1741 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt 1801 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg 1861 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc 1921 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca 1981 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag 2041 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa 2101 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac 2161 agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag 2221 cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 2401 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 2461 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 2521 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 2641 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga 2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga ctctagaatg 2821 tcaagctctt cctggctcct tctcagcctt gttgctgtaa ctgctgctca gtccaccatt 2881 gaggaacagg ccaagacatt tttggacaag tttaaccacg aagccgaaga cctgttctat 2941 caaagttcac ttgcttcttg gaattataac accaatatta ctgaagagaa tgtccaaaac 3001 atgaataatg ctggggacaa atggtctgcc tttttaaagg aacagtccac acttgcccaa 3061 atgtatccac tacaagaaat tcagaatctc acagtcaagc ttcagctgca ggctcttcag 3121 caaaatgggt cttcagtgct ctcagaagac aagagcaaac ggttgaacac aattctaaat 3181 acaatgagca ccatctacag tactggaaaa gtttgtaacc cagataatcc acaagaatgc 3241 ttattacttg aaccaggttt gaatgaaata atggcaaaca gtttagacta caatgagagg 3301 ctctgggctt gggaaagctg gagatctgag gtcggcaagc agctgaggcc attatatgaa 3361 gagtatgtgg tcttgaaaaa tgagatggca agagcaaatc attatgagga ctatggggat 3421 tattggagag gagactatga agtaaatggg gtagatggct atgactacag ccgcggccag 3481 ttgattgaag atgtggaaca tacctttgaa gagattaaac cattatatga acatcttcat 3541 gcctatgtga gggcaaagtt gatgaatgcc tatccttcct atatcagtcc aattggatgc 3601 ctccctgctc atttgcttgg tgatatgtgg ggtagatttt ggacaaatct gtactctttg 3661 acagttccct ttggacagaa accaaacata gatgttactg atgcaatggt ggaccaggcc 3721 tgggatgcac agagaatatt caaggaggcc gagaagttct ttgtatctgt tggtcttcct 3781 aatatgactc aaggattctg ggaaaattcc atgctaacgg acccaggaaa tgttcagaaa 3841 gcagtctgcc atcccacagc ttgggacctg gggaagggcg acttcaggat ccttatgtgc 3901 acaaaggtga caatggacga cttcctgaca gctcatcatg agatggggca tatccagtat 3961 gatatggcat atgctgcaca accttttctg ctaagaaatg gagctaatga aggattccat 4021 gaagctgttg gggaaatcat gtcactttct gcagccacac ctaagcattt aaaatccatt 4081 ggtcttctgt cacccgattt tcaagaagac aatgaaacag aaataaactt cctgctcaaa 4141 caagcactca cgattgttgg gactctgcca tttacttaca tgttagagaa gtggaggtgg 4201 atggtcttta aaggggaaat tcccaaagac cagtggatga aaaagtggtg ggagatgaag 4261 cgagagatag ttggggtggt ggaacctgtg ccccatgatg aaacatactg tgaccccgca 4321 tctctgttcc atgtttctaa tgattactca ttcattcgat attacacaag gaccctttac 4381 caattccagt ttcaagaagc actttgtcaa gcagctaaac atgaaggccc tctgcacaaa 4441 tgtgacatct caaactctac agaagctgga cagaaactgt tcaatatgct gaggcttgga 4501 aaatcagaac cctggaccct agcattggaa aatgttgtag gagcaaagaa catgaatgta 4561 aggccactgc tcaactactt tgagccctta tttacctggc tgaaagacca gaacaagaat 4621 tcttttgtgg gatggagtac cgactggagt ccatatgcag accaaagcat caaagtgagg 4681 ataagcctaa aatcagctct tggagataaa gcatatgaat ggaacgacaa tgaaatgtac 4741 ctgttccgat catctgttgc atatgctatg aggcagtact ttttaaaagt aaaaaatcag 4801 atgattcttt ttggggagga ggatgtgcga gtggctaatt tgaaaccaag aatctccttt 4861 aatttctttg tcactgcacc taaaaatgtg tctgatatca ttcctagaac tgaagttgaa 4921 aaggccatca ggatgtcccg gagccgtatc aatgatgctt tccgtctgaa tgacaacagc 4981 ctagagtttc tggggataca gccaacactt ggacctccta accagccccc tgtttccata 5041 tggctgattg tttttggagt tgtgatggga gtgatagtgg ttggcattgt catcctgatc 5101 ttcactggga tcagagatcg gaagaagaaa aataaagcaa gaagtggaga aaatccttat 5161 gcctccatcg atattagcaa aggagaaaat aatccaggat tccaaaacac tgatgatgtt 5221 cagacctcct ttggtaccga gacctcccag gtggcgcccg cttaaactag ttaacgcgtc 5281 gaccagtggt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc 5341 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag 5401 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 5461 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct 5521 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 5581 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt 5641 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 5701 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 5761 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 5821 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 5881 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 5941 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 6001 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 6061 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 6121 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 6181 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 6241 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg 6301 ccagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 6361 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 6421 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 6481 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 6541 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 6601 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 6661 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 6721 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 6781 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 6841 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct 6901 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 6961 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc 7021 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 7081 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 7141 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 7201 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 7261 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 7321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 7381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 7441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 7501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa 7561 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg 7621 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 7681 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 7741 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc 7801 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 7861 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 7921 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 7981 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 8041 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 8101 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 8161 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 8221 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa 8281 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 8341 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 8401 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 8461 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 8521 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 8581 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 8641 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 8701 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 8761 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 8821 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 8881 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 8941 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 9001 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 9061 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 9121 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 9181 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 9241 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 9301 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 9361 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 9421 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 9481 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 9541 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 9601 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 9661 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 9721 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 9781 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 9841 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 9901 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 9961 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 10021 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 10081 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 10141 cgaccgagcg cagcgagtca gtgagcgagg aag //