LOCUS       Exported                4296 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 nsp11.
ACCESSION   .
VERSION     .
KEYWORDS    pGBW-m4133196
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4296)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    182..230
                     /note="synthetic polyadenylation signal"
     terminator      248..294
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    299..370
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      522..658
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(671..1531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1294..1313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2277..2865
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2766..2785
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3091..3162
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      3207..3343
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      3373..3419
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    3534..3655
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3571..3590)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3625..3644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3708..3752)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(3753..3773)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     promoter        complement(3890..4092)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3919..3939)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4094..101
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
       61 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
      121 tatgaactaa tgaccccgta attgattact agatctggct acactcgatc agcagttaga
      181 taataaaatc gctatccatc gaagatggat gtgtgttggt tttttgtgtg tgtaacgcaa
      241 cgattgatag cataacccct tggggcctct aaacgggtct tgaggggttt tttgaattga
      301 gaggtagctg aagctgctgt tatgaccact agagggaaga agatacctgt ggagctaatg
      361 gtccaagatg tcgggagccg cgatcgctgc caaaaaggta agctgacgat agctgacgag
      421 attcgatgcc cgacgagcaa atgagcgcca cggctgttcg cgtaagctcg aaacgacgga
      481 gattcgatag agccagagtc gagtttaaac gattcgggat cttaaggata aatttctggt
      541 aaggaggaca cgtatggaag tgggcaagtt ggggaagccg tatccgttgc tgaatctggc
      601 atatgtggga gtataagacg cgcagcgtcg catcaggcat ttttttctgc gccaatgcac
      661 ctagaatcgg ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
      721 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
      781 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
      841 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
      901 cctccatcca gtctatcaac tgttgccggg aagctagagt aagtagttcg ccagttaata
      961 gtttacgcaa ggttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1021 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1081 gcaaaaaagc ggttagctcc ttcggtcctc caatcgttgt cagaagtaag ttggccgcag
     1141 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1201 gatgcttttc tgtgactggt gaatactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1261 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1321 taaaagtgct catcattgga aagcgttctt cggggcgaaa actctcaagg atcttaccgc
     1381 tgttgagatc cagttcgatg taacccactc gcgcacccaa ctgatcttca gcatctttta
     1441 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1501 taagggcgac acggaaatgt tgaatactca tgcgaaagcc tcctgctgat cctgtctctt
     1561 gatagatgtt tgcaaaagct aggactcgat tatacgttga ccgatcagac tatcaatatc
     1621 taggtttaaa cagatcggta cgctgccgtg accaggcttg agtacaatat ctgacgattg
     1681 gacttcctgg gtcccaagct ggattccgac tggctgactg ccaagcaacg atatggatcg
     1741 tcgagactcg cactgagagg atcgttctcg gtacaccagg cgcgccccga tttcgatatt
     1801 cctcggatct cggatagatc gggattgagg atgtaggttg aacttctctc gggtgatccc
     1861 gatctcggat attcgggatt ggagtaatac ctggcgatag gcgctgccaa gcaatagcca
     1921 aggcgcaagg ccggccaaga gacttgcgat agttcgttcg agaggtagcc tgcaagatgg
     1981 atcggacgaa aggtttgaag attttccgac gagggccttg gagagttcgg aaatttaaat
     2041 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2101 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2161 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2221 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2281 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2341 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2401 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2461 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2521 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2581 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2641 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2701 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2761 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2821 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2881 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgattta aatgaaactc
     2941 gtaatcggag aacgggtgca atagcaagga ccagatcgaa cttgactgac gcaattctga
     3001 gcgtagtcga aacccagaca agtcgatctc gccaagtagc tgacttgact ggttaattaa
     3061 gtttcgatag cccaaggtaa ccaatgagat tagggaggga agaaagtttg atgaactact
     3121 tctgacccct agtggtgtcc agaaaagacc attaaaggaa tgtcttgatc gaaatcgaga
     3181 gatcgcctgc aggatagctg acgaccttaa ggataaattt ctggtaagga ggacacgtat
     3241 ggaagtgggc aagttgggga agccgtatcc gttgctgaat ctggcatatg tgggagtata
     3301 agacgcgcag cgtcgcatca ggcatttttt tctgcgccaa tgccggtaga tagcaatccg
     3361 gcccgagggg cacaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctac
     3421 gataccgata gagatgggcc aataccacat ttgtagaggt tttacttgct ttaaaaaacc
     3481 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     3541 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3601 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3661 tctgtgagct gaaggtacgc tgtatctcag tcagtcaagc tagcttaact gtccatgtgc
     3721 tgcctctcga acttagctgc cgcggtttct ttcgactgaa agtaaaggtt ttccactgcg
     3781 aaaccgttga gaaaggactg agcatcggca ctcatggtgg cggccgctgg cgggtgtgtc
     3841 gagtggatgg taggatcgac aaagatctga tctgacggtt cactaaacca gctctgctta
     3901 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     3961 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     4021 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     4081 aaaaccgcat cagcatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     4141 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     4201 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     4261 gtaaatactc cacccattga cgtcaatgga aagtcc
//
