LOCUS Exported 7242 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site. ACCESSION . VERSION . KEYWORDS pCRISPR2_+1D SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7242) TITLE Phasing paper plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 7242) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7242 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 1874..1896 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" promoter complement(1923..2207) /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind complement(1981..2000) /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" CDS 2234..3112 /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" oriT 3269..3377 /note="incP origin of transfer" rep_origin complement(3447..4035) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3527..3546) /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter complement(5539..5567) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" rep_origin 5608..5959 /label=pRO1600 oriV /note="broad-host-range origin of replication from Pseudomonas aeruginosa plasmid pRO1600; requires the pRO1600 Rep protein for replication (West et al., 1994)" CDS 5973..6806 /codon_start=1 /product="replication protein for the broad-host-range plasmid pRO1600 from Pseudomonas aeruginosa " /label=pRO1600 Rep /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA" primer_bind 6952..6974 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6966..6983 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 6967..6983 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gggtggttct gcctagacgg cagcgaaccc ggcggctgtt gtggccgagg tgggttcgag 61 tttcttagct gcctacacgg cagtgaactt gacgaacgcc gtccagaagt caccacccgg 121 ctttcttagc tgcctacacg gcagtgaaca ccgcgctcga ctactacaac gtccggctga 181 ttttcttagc tgcctacacg gcagtgaact agctccgaaa acctaccggc ggctgttgtg 241 gccgaggtgg gttcgagttt cttagctgcc tacacggcag tgaactggat tcatgtatca 301 ggcatcgtac tcggcaattt cttagctgcc tacacggcag tgaaccaggt agacgcggac 361 atcaagcccg ccgtgaattt cttagctgcc tacacggcag tgaacgagag ccgcagggag 421 cggggccgca aactcctttt cttagctgcc tacacggcag tgaactggag gcgaccgaac 481 ccgggctgtt cgaggatttt cttagctgcc tacacggcag tgaacaaccg cgcgcaacag 541 ctgtgcatcg tcaccttttt cttagctgcc tacacggcag tgaaccgtct cgatgctgcc 601 gtcgatgagc gtcttgtttt cttagctgcc tacacggcag tgaacttatc aagcggctgt 661 gctgggcctg tttcgttttt cttagctgcc tacacggcag tgaacgcctt cggtggtgat 721 ctttccgttg tttgcctttt cttagctgcc tacacggcag tgaacaatcg tgctcgagat 781 accagccatc cgcgcacttt cttagctgcc tacacggcag tgaacaacag aaccgcgcac 841 ctcgatcagc ctcgcatttt cttagctgcc tacacggcag tgaacgtaaa tccagcgcct 901 gggctgcgca ttgtccattt cttagctgcc tacacggcag tgaaccacgc agccaggctc 961 gaacatcggc ccggcttttt cttagctgcc tacacggcag tgaactacta cgacgacgct 1021 ttgcaggctg atattcattt cttagctgcc tacacggcag tgaacacgac gcgaccagaa 1081 tcgcgatttg cacccacttt cttagctgcc tacacggcag tgaacgcgct gacgccgcat 1141 tcggcgatct gcttcgtttt cttagctgcc tacacggcag tgaaccgggc gcttgcgccg 1201 gatcggcgaa ttggatgttt cttagctgcc tacacggcag tgaacccggg ctggatggac 1261 tcgaagggcg ccaggctttt cttagctgcc tacacggcag tgaacgacca gatcctgccg 1321 cagtactggc aggaccgttt cttagctgcc tacacggcag tgaacgtcgc agccactgtg 1381 tcggccaaaa ccaggtcttt cttagctgcc tacacggcag tgaacttgac gaacgccgtc 1441 cagaagtcac cacccggctt tcttagctgc ctacacggca gtgaacaccg cgctcgacta 1501 ctacaacgtc cggctgattt tcttagctgc ctacacggca gtgaactagc tccgaaaacc 1561 tactaaccat ttgaaggaaa aagaaatacc gagtttttcc cgccagaaac cctctttttt 1621 cgaggtctcg taacttgctg atttataagg gcgtaagaaa tcgtccgaaa aaagggtcgg 1681 aagcttcggg caattggcgg ccgtttcgcg aggaccggga cggcgatcaa ccgtgccgtt 1741 cgggcgcgaa aggtcttttg atgggcgatg ccgcaaagcc cccggccagg ccgggggcgt 1801 cgcgccaggg agcggcgcta caggctcggg tggcccgcct cgatttcgga caaggcatcc 1861 atgggtatgt atatctcctt cttaaagtta aacaaaatta tttctagccc aaaaaaacgg 1921 gtatggagaa acagtagaga gttgcgataa aaagcgtcag gtaggatccg ctaatcttat 1981 ggataaaaat gctatggcat agcaaagtgt gacgccgtgc aaataatcaa tgtggacttt 2041 tctgccgtga ttatagacac ttttgttacg cgtttttgtc atggctttgg tcccgctttg 2101 ttacagaatg cttttaataa gcggggttac cggtttggtt agcgagaaga gccagtaaaa 2161 gacgcagtga cggcaatgtc tgatgcaata tggacaattg gtttcttctc tgaatggcgg 2221 gagtatgaaa agtatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa 2281 tgcccatctg gtggcgggtt taacgccgat tgaggccaac ggttatctcg atttttttat 2341 cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggggt 2401 ggtgaaaaat cagggacgag aatttgtttg ccgaccgggt gatattttgc tgttcccgcc 2461 aggagagatt catcactacg gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt 2521 ttactttcgt ccgcgcgcct actggcatga atggcttaac tggccgtcaa tatttgccaa 2581 tacggggttc tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca 2641 aatcattaac gccgggcaag gggaagggcg ctattcggag ctgctggcga taaatctgct 2701 tgagcaattg ttactgcggc gcatggaagc gattaacgag tcgctccatc caccgatgga 2761 taatcgggta cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gcaattttga 2821 tatcgccagc gtcgcacagc atgtttgctt gtcgccgtcg cgtctgtcac atcttttccg 2881 ccagcagtta gggattagcg tcttaagctg gcgcgaggac caacgtatca gccaggcgaa 2941 gctgcttttg agcaccaccc ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga 3001 cgatcaactc tatttctcgc gggtatttaa aaaatgcacc ggggccagcc cgagcgagtt 3061 ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcat aatttgggga 3121 ttccttaagg tatactttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt 3181 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact 3241 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga 3301 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg 3361 ctctgcgagg ctggccgata agctagcttg tgagcaaaag gccagcaaaa ggccaggaac 3421 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 3481 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 3541 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 3601 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 3661 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 3721 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 3781 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 3841 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt 3901 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 3961 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 4021 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 4081 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 4141 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 4201 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 4261 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 4321 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 4381 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 4441 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 4501 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 4561 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 4621 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 4681 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 4741 ttttctgtga ctggtgagtc gaattggccg cggcgttgtg acaatttacc gaacaactcc 4801 gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact tgggtcgata 4861 tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac tgcgggatcg 4921 tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc ctcatgcttg 4981 aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga gactgcgaga 5041 tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta agccgcgaga 5101 gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt actacggagc 5161 aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac gtctccgaac 5221 tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg gccgagccta 5281 catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg actgccctgc 5341 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc 5401 tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc catgaaaacc 5461 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc 5521 atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaattc gcgccgctgg 5581 tgccgctggt tggacgccaa gggtgaatcc gcctcgatac cctgattact cgcttcctgc 5641 gccctctcag gcggcgatag gggactggta aaacggggat tgcccagacg cctcccccgc 5701 cccttcaggg gcacaaatgc ggccccaacg gggccacgta gtggtgcgtt ttttgcgttt 5761 ccaccctttt cttccttttc ccttttaaac cttttaggac gtctacaggc cacgtaatcc 5821 gtggcctgta gagtttaaaa agggacggat ttgttgccat taagggacgg atttgttgtt 5881 aagaagggac ggatttgttg ttgtaaaggg acggatttgt tgtattgtgg gacgcagata 5941 cagtgtcccc ttatacacaa ggaatgtcga acgtggcctc acccccaatg gtttacaaaa 6001 gcaatgccct ggtcgaggcc gcgtatcgcc tcagtgttca ggaacagcgg atcgttctgg 6061 cctgtattag ccaggtgaag aggagcgagc ctgtcaccga tgaagtgatg tattcagtga 6121 cggcggagga catagcgacg atggcgggtg tccctatcga atcttcctac aaccagctca 6181 aagaagcggc cctgcgcctg aaacggcggg aagtccggtt aacccaagag cccaatggca 6241 aggggaaaag accgagtgtg atgattaccg gctgggtgca aacaatcatc taccgggagg 6301 gtgagggccg tgtagaactc aggttcacca aagacatgct gccgtacctg acggaactca 6361 ccaaacagtt caccaaatac gccttggctg acgtggccaa gatggacagc acccacgcga 6421 tcaggcttta cgagctgctc atgcaatggg acagcatcgg ccagcgcgaa atagaaattg 6481 accagctgcg aaagtggttt caactggaag gccggtatcc ctcgatcaag gacttcaagt 6541 tgcgagtgct tgatccagcc gtgacgcaga tcaacgagca cagcccgcta caggtggagt 6601 gggcgcagcg aaagaccggg cgcaaggtca cacatctgtt gttcagtttt ggaccgaaga 6661 agcccgccaa ggcggtgggt aaggccccag cgaagcgcaa ggccgggaag atttcagatg 6721 ctgagatcgc gaaacaggct cgccctggtg agacatggga agcggcccgc gctcgactaa 6781 cccagatgcc gctggatctg gcctagaggc cgtggccacc acggcccggc ctgcctttca 6841 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 6901 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 6961 gacgttgtaa aacgacggcc agtgccaagc ttgcatgcga gtgaaggatc gtcttcagtt 7021 cgctgggaga aatgtcgtcc atgttgaccg tggcctgtcc ctatcggatc tatcgaattc 7081 tgcgccaagg caactgttta gcggatatgg ccgattgccg ctaccccgtt ttcgtctgtg 7141 tgaggagcgt gagcttccaa gcgaggcaca ttgtcgtgga cgggcgaggc tggcgggcgg 7201 ggtaggaact ttatattcgg atgtttggta gtgggatggg ga //