LOCUS Exported 11244 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Express NSP14 gene along with HA and BASU. ACCESSION . VERSION . KEYWORDS SARS_COV2_nsp14_HA_BASU SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11244) TITLE Khavari lab SARS-CoV-2 plasmids REFERENCE 2 (bases 1 to 11244) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11244 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 1..634 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 681..806 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1303..1536 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1721..1765 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2028..2143 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" promoter 2338..3519 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 2568..3510 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 3467..3487 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" CDS 3553..3579 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 4366..4389 /codon_start=1 /product="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" /label=Strep-Tag II /translation="WSHPQFEK" misc_feature 5994..6567 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(6161..6178) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 6388..6407 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 6600..7199 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(6600..6619) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 7096..7116 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 7213..7801 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7266..7286) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7684..7695) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8008..8641 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" primer_bind complement(8769..8785) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8769..8785) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8782..8804) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8793..8809 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8817..8847) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8862..8883 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9000..9017) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9171..9759) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9251..9270) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9930..10790) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10553..10572 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10791..10895) /gene="bla" /label=AmpR promoter polyA_signal 10943..11077 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10980..10999) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 11034..11053 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc 3541 gccgccacca tgtacccata cgatgtgcct gactatgctg gcaagttgtc tgaatcagag 3601 attaggtttg gactgaaaac ggaggtgatg ggccaacatc ttatctatca cgatgttttg 3661 tccagcacac aaaagacagc ccatgaattg gcaaataaca atgcccccga gggtaccctc 3721 gtggttgcag ataaacagac agccggtagg ggcgggatga gccgagtgtg gcattctcaa 3781 gaaggaaacg gagtatggat gagtttgata ttgcgcccgg acattccgct ccaaaagacg 3841 ccccaactta ccttgctggc agctgtagcg gtggttcagg gaatagaaga ggccgcgggc 3901 atacagactg atataaagtg gccgaacgac atactcatta acgggaaaaa aacagtaggg 3961 atacttaccg aaatgcaagc tgaggaggat cgagtgcgct ccgtgattat tggtattggc 4021 attaatgtaa atcagcaacc caatgatttc cccgatgagc tcaaggacat tgcgacgagt 4081 ctgtcccagg cagctgggga aaagatcgac agggcaggtg taatccagca tatcttgctt 4141 tgtttcgaaa agcgataccg agactacatg acccacggtt tcacgcccat caagctcttg 4201 tgggagtcat acgccctcgg tattggcacg aacatgaggg cgagaactct caacgggaca 4261 ttttacggta aggcgttggg tattgacgac gaaggggtac ttttgttgga aactaatgaa 4321 ggcattaaga agatatacag cgcggacatc agccttaggg ggagctggtc tcacccgcaa 4381 tttgaaaaag gcgctgcggc tgctgaaaat gtaacgggct tgtttaaaga ctgtagtaaa 4441 gtgatcacag gactccaccc cacacaagca cctacccacc tttccgtaga tacgaagttc 4501 aaaacggaag gattgtgtgt ggatatacca gggataccaa aggatatgac gtaccgaagg 4561 ctgatttcca tgatgggttt taagatgaat taccaagtta atggctaccc caacatgttc 4621 atcaccaggg aggaggcaat tagacacgta agagcctgga taggcttcga tgttgaaggt 4681 tgccatgcaa cccgggaagc cgtaggcaca aaccttccgt tgcagcttgg cttttccacg 4741 ggcgtcaacc tcgttgccgt accgactggc tatgttgaca cgccgaacaa caccgatttc 4801 tctcgcgtaa gtgctaagcc tcctccggga gatcaattca agcatcttat acctctcatg 4861 tacaagggac tgccgtggaa tgtagttcgc attaagatcg tgcagatgct ctccgatact 4921 ttgaaaaatc tgagcgacag ggtggtcttc gttctgtggg cacatggctt cgaacttact 4981 tctatgaaat actttgtaaa aatcgggccc gagcggacat gttgtttgtg cgaccgacga 5041 gctacttgtt ttagcacagc atctgacacc tacgcatgct ggcaccacag tataggcttc 5101 gattacgtct acaatccctt tatgatagac gtacaacaat ggggctttac gggtaacttg 5161 cagagtaatc acgatcttta ctgccaagtt catgggaacg cacacgtggc ctcctgcgat 5221 gcgataatga cgaggtgctt ggccgtgcac gagtgctttg ttaagcgggt cgattggact 5281 atagagtatc ccataatcgg tgacgaactt aaaattaatg ctgcatgcag gaaagtgcag 5341 cacatggtag taaaagcagc gctcctcgcg gataagtttc ctgtgctcca cgatatcggc 5401 aaccctaagg ccattaaatg cgtcccccaa gctgatgtag agtggaaatt ctatgacgct 5461 cagccatgta gtgacaaagc atacaagatt gaagaattgt tttattcata tgcgacacac 5521 tccgataagt tcactgacgg agtttgtctc ttttggaact gtaatgttga taggtatccg 5581 gcgaatagca tagtgtgtcg atttgacacg cgcgttctct ctaatttgaa cctccctggc 5641 tgcgacgggg ggtctttgta cgtaaacaag catgcatttc ataccccagc ttttgacaaa 5701 tccgctttcg taaatctgaa acaacttccc ttcttctatt attcagatag cccttgcgaa 5761 tctcacggta agcaagtggt tagtgacatt gactatgtac cacttaaaag tgcaacgtgc 5821 attacacgat gcaatttggg gggcgcggtt tgccgacacc atgccaacga atacagattg 5881 tacctggatg catataacat gatgataagt gcagggttta gtctttgggt gtataagcag 5941 ttcgatacgt ataatctctg gaacacattt acccgattgc agtagggatc ccgcccctct 6001 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt 6061 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct 6121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa 6181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg 6241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc 6301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg 6361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg 6421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc 6481 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg 6541 gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga cgaccttcca 6601 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac 6661 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc 6721 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 6781 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 6841 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 6901 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 6961 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 7021 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 7081 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 7141 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaga 7201 cgcgtctgga acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt 7261 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 7321 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt 7381 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac 7441 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct 7501 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca 7561 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt 7621 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 7681 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct 7741 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg 7801 cctggaatta attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata 7861 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt 7921 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta 7981 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag 8041 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca 8101 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag 8161 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg 8221 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc 8281 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat 8341 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg 8401 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact 8461 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 8521 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 8581 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 8641 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 8701 gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg 8761 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 8821 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 8881 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 8941 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 9001 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 9061 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 9121 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 9181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 9241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 9301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 9361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 9421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 9481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 9541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 9601 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 9661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 9721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 9781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 9841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 9901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 9961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 10021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 10081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 10141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 10201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 10261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 10321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 10381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 10441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 10501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 10561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 10621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 10681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 10741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 10801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 10861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 10921 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag 10981 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 11041 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca 11101 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca 11161 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta 11221 aatatgtact acaaacttag tagt //